We narrowed to 10,757 results for: ESP

Showing: 4001 - 4050 of 10757 results

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Mutation

R262W

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti

Plasmid

#218657

Purpose

Knockout vector for human PLEKHA3 (FAPP1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA3 (PLEKHA3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

Plasmid

#218656

Purpose

Knockout vector for human PLEKHA3 (FAPP1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA3 (PLEKHA3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ318

Plasmid

#217495

Purpose

Mammlian expression of MmFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

MmFnuc guide RNA scaffold

Expression

Mammalian

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ234

Plasmid

#217492

Purpose

Mammlian expression of DpFnuc for genome editing

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ221

Plasmid

#212319

Purpose

Express MmFnuc guide with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria guide

Expression

Bacterial

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ238

Plasmid

#212321

Purpose

Express KnFnuc guide with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFunc guide

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep

Plasmid

#213542

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 Orf3a-2xStrep (ORF3a Human, Severe acute respiratory syndrome coronavirus 2)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep

Plasmid

#213543

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 protE-2xStrep (E Human, Severe acute respiratory syndrome coronavirus 2)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204786

Purpose

Bacterial expression of Sars Cov 2 MPRO T190I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

T190I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204784

Purpose

Bacterial expression of Sars Cov 2 MPRO Q189K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

Q189K

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204783

Purpose

Bacterial expression of Sars Cov 2 MPRO P132H mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

P132H

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204782

Purpose

Bacterial expression of Sars Cov 2 MPRO N142S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

N142S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204779

Purpose

Bacterial expression of Sars Cov 2 MPRO M49T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204777

Purpose

Bacterial expression of Sars Cov 2 MPRO M49I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204776

Purpose

Bacterial expression of Sars Cov 2 MPRO G143S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

G143S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204774

Purpose

Bacterial expression of Sars Cov 2 MPRO A191V mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191V

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204773

Purpose

Bacterial expression of Sars Cov 2 MPRO A193T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A191T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204772

Purpose

Bacterial expression of Sars Cov 2 MPRO A191T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A193S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204771

Purpose

Bacterial expression of Sars Cov 2 MPRO A193S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-ApaLIE225A, K227A-HA

Plasmid

#211460

Purpose

Express mitochondrial localized ApaLI with E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-E225A-K227A

Tags

HA

Expression

Mammalian

Mutation

E225A, K227A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ217

Plasmid

#212318

Purpose

Express MmFnuc with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCALPS-EGFP-Cre

Plasmid

#207132

Purpose

Lentiviral vector to express EGFP-Cre

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

EGFP-Cre

Use

Lentiviral

Promoter

SSFV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

C145S mutant

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDRF1-GW ymTq2-d11

Plasmid

#205490

Purpose

express ymTq2-d11 as donor control for yAT1.03

Depositor

Bas Teusink

Article

Luzia et al iScience. 2023 Dec 21;27(1):108767. doi:

Insert

ymTq2-d11

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p32m3C Trx-CN

Plasmid

#196474

Purpose

Protein expression of Thiolredoxin-3C cleavage site-CarHCN

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

Trx-CN

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-mtagBFP-2A-ZebrafishSTING

Plasmid

#196633

Purpose

Express Zebrasfish STING

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

ZebrafishSTING (sting1 Zebrafish)

Use

Lentiviral

Tags

mtagBFP

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

Plasmid

#173122

Purpose

Expresses uTEV1 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV1-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG CAG CCL20-pre-mGRASP

Plasmid

#173114

Purpose

Expresses CCL20 sender construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 CMV myc-Arrestin-TEVp

Plasmid

#173115

Purpose

Expresses arrestin receiver construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

myc-Arrestin-TEVp

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

Plasmid

#173121

Purpose

Expresses TEVp(WT) and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp(WT)-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS306-PTDH3-Dimer-1SUMO

Plasmid

#190249

Purpose

Expression dimer-1SUMO component in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

dimer-SUMO

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS306-PTDH3-SUMO7-6SIM

Plasmid

#190250

Purpose

Expression SUMO7-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

7SUMO-6SIM

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS304-PTDH3-HEXAMER

Plasmid

#190251

Purpose

Expression hexamer component in yeast

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

hexamer

Tags

mCherry

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP8GF-AtAFB2

Plasmid

#188426

Purpose

Arabidopsis Nuclear Auxin Signaling Receptor AFB2

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-ZmIAA25

Plasmid

#188439

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA25

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA25

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-ZmIAA8

Plasmid

#188431

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA8

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA8

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-ZmIAA16

Plasmid

#188437

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA16

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA16

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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