We narrowed to 40,827 results for: KAN

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pBC080

Plasmid

#202239

Purpose

Level 2 for strain-specific barcode DNA tag with bc107 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc107

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC081

Plasmid

#202240

Purpose

Level 2 for strain-specific barcode DNA tag with bc108 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc108

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC082

Plasmid

#202241

Purpose

Level 2 for strain-specific barcode DNA tag with bc109 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc109

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC083

Plasmid

#202242

Purpose

Level 2 for strain-specific barcode DNA tag with bc110 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc110

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC104

Plasmid

#202243

Purpose

Level 2 for strain-specific barcode DNA tag with bc131 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc131

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC105

Plasmid

#202244

Purpose

Level 2 for strain-specific barcode DNA tag with bc132 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc132

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC106

Plasmid

#202245

Purpose

Level 2 for strain-specific barcode DNA tag with bc133 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc133

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC107

Plasmid

#202246

Purpose

Level 2 for strain-specific barcode DNA tag with bc134 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc134

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-NlaCas7-p65

Plasmid

#207394

Purpose

Expresses NlaCas7-p65 activator fusion in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-NlaCas7-p65-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEN-X-A-ccdB-G-Z

Plasmid

#218892

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

Plasmid

#246754

Purpose

RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

Use

Synthetic Biology

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

Plasmid

#246755

Purpose

RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

Use

Synthetic Biology

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRelA*

Plasmid

#175590

Purpose

Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Article

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Catalytic domain of E.coli relA gene.

Tags

Fused with a flexible GS linker to mVenus

Expression

Bacterial

Mutation

Only the catalytic domain of the enzyme is used (…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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His-DNPH1

Plasmid

#203224

Purpose

For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

Depositor

Vern Schramm

Article

Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

Insert

DNPH1 (DNPH1 Human)

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI

Plasmid

#246757

Purpose

RNA circularization with CIRC using Azo intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI

Use

Synthetic Biology

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW90004

Plasmid

#222436

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00011

Plasmid

#222439

Purpose

Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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