We narrowed to 4,318 results for: Pet2

1. pET23b-Casp9 C287A-His

   Plasmid

   #11830

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

   Insert

   Caspase 9 catalytic mutant (CASP9 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C287A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET21b GFP-Rab10 Q68LHis

   Plasmid

   #186015

   Purpose

   GFP-Rab10 Q68LHis

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   GFP-Rab10 Q68LHis (RAB10 Human)
   

   Expression

   Bacterial

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-HsSENP3(307-574)

   Plasmid

   #71481

   Purpose

   Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-306 (catalytic domain only)

   Promoter

   T7

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET22-6His-SUMO-Nsp10

   Plasmid

   #154960

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-his-Gluc-iLID

   Plasmid

   #172096

   Purpose

   Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

   Depositor

   Avi Schroeder

   Article

   Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

   Insert

   Gaussia Luciferase - iLID
   

   Expression

   Bacterial

   Mutation

   Gluc Mutations - M43L, M110L

   Promoter

   T7

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24a-VHH-tev-APEX2

   Plasmid

   #117751

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-his6-HA3-CLIP

   Plasmid

   #188942

   Purpose

   T7 expression vector for making his6- and HA3-tagged CLIP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-CLIP
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet22b-mEGFP-YAP-N-IDR

   Plasmid

   #166440

   Purpose

   Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAP-N-IDR(1-164) (YAP1 Human)
   

   Expression

   Bacterial

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Hook3 aa 1-160-GCN4

    Plasmid

    #74608

    Purpose

    Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Tags

    6x His, GCN4, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23b-Casp7 C186A-His

    Plasmid

    #11826

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 7 catalytic mutant (CASP7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C186A

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

12. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pET28-sfGFP[D134TAG+N150TAA]

    Plasmid

    #164582

    Purpose

    superfolder GFP with D134TAG and N150TAA mutations

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-134TAG+150TAA
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAA Mutations

    Promoter

    T7

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a(+) 6xHis-TEV-IFIT5

    Plasmid

    #53560

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-Hisx6-Sirt6-H133Y

    Plasmid

    #20279

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Histidine 133 to Tyrosine

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pET28-Strep-TEV-Nsp14

    Plasmid

    #154955

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    Strep-TEV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET28-His6-Ubc9-C93A

    Plasmid

    #133910

    Purpose

    Bacterial expression of His6-Ubc9-C93A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 93 to Alanine

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b(+)_S. cerevisiae Hbs1(1-611)

    Plasmid

    #39336

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET28a-Gluc-EL222-his

    Plasmid

    #172097

    Purpose

    Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

    Depositor

    Avi Schroeder

    Article

    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

    Insert

    Gaussia Luciferase - EL222
    

    Expression

    Bacterial

    Mutation

    Gluc mutations - M43L, M110L

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge