We narrowed to 62,126 results for: cloning
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Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone
Plasmid#228362PurposemU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.DepositorTypeEmpty backboneUseCRISPR and Lentiviral; Rfxcas13d grna expression …ExpressionMammalianPromotermU6 and hU6Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB1: All in one SpCas9 expression and sgRNA cloning backbone
Plasmid#228363PurposemU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherryDepositorTypeEmpty backboneUseCRISPR and Lentiviral; Spcas9 nuclease and spcas9…ExpressionMammalianPromotermU6Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG022
Plasmid#231306PurposeVector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpRDepositorInsertBEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)
Plasmid#55213DepositorTypeEmpty backboneTags6xHis, High pI protein 85, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)
Plasmid#48290DepositorTypeEmpty backboneTagsStrepIIExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)
Plasmid#48286DepositorTypeEmpty backboneTagsHis6 and MBPExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)
Plasmid#48287DepositorTypeEmpty backboneTagsHis6 and msfGFPExpressionBacterialAvailable SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
Plasmid#105922PurposeAAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITRDepositorInsertsmKate2
IRES-Multiple cloning site
UseAAVMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available SinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBZCas13b
Plasmid#89898PurposeBacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-mCherry
Plasmid#185474PurposeMammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 High pI protein 1 TEV LIC cloning vector (2-HpI-1)
Plasmid#55210DepositorTypeEmpty backboneTags6xHis, High pI protein 1, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 High pI protein 68 TEV LIC cloning vector (2-HpI-68)
Plasmid#55211DepositorTypeEmpty backboneTags6xHis, High pI protein 68, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorInsertPE7-P2A-EGFP
TagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianPromoterCMVAvailable SinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
M23: pHR-SFFVp-FUSN::mCherry::SspB
Plasmid#122148PurposeN terminal domain of FUS (1-214) tagged with SspB and mCherry.DepositorInsertFUS (FUS Human)
UseLentiviralTagsmCherry-sspBExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-ERT2-Cre-ERT2
Plasmid#82587PurposeMiddle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombinationDepositorInsertERT2-CRE-ERT2
PromoterNoneAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-GFP-LOVpep
Plasmid#80406PurposeMembrane localized GFP-tagged LOVpep. Expresses in mammalian cellsDepositorAvailable SinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GAL9
Plasmid#166689PurposeFor creating a stable mCherry-GAL9 cell lineDepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPPromoterCMV immediate earlyAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only