Addgene

We narrowed to 3,518 results for: mak

Showing: 1601 - 1620 of 3518 results

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pLenti-hSyn-eNpHR 3.0-EYFP

Plasmid

#26775

Purpose

3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Insert

eNpHR 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

hSyn

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 K82R (Nigg LA184)

Plasmid

#41157

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K82R

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pET22b-His6-UBCH7

Plasmid

#212711

Purpose

Bacterial expression of His6-UBCH7

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

UBCH7

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-3xMyc-STX17FL

Plasmid

#89942

Purpose

Expresses syntaxin17 tagged with Myc in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 (STX17 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL_U2AF1_S34Y_mCherry

Plasmid

#84018

Purpose

expression of mutant U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

S34Y

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pGBW-m4137384

Plasmid

#149543

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)

Use

Tags

Expression

Mammalian

Mutation

H. sapiens recode

Promoter

CMV

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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plYJP022-int7

Plasmid

#202287

Purpose

Plasmid containing integrase for LPattB7 site

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

int7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PARP9-c020

Plasmid

#173097

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP9 (PARP9 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

N310-N493

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pSC12

Plasmid

#104786

Purpose

pNB184-Cas9 entry cassette

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSC10

Plasmid

#104784

Purpose

pAH595-gRNA 2xplex entry cassette

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJH001

Plasmid

#67641

Purpose

High copy Cas9 expression vector with LEU2 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
Empty

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGBW-m4137382

Plasmid

#149539

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)

Use

Tags

Expression

Mammalian

Mutation

H. sapiens recode;19-aa Ctrunc

Promoter

CMV

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Use

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFBD-SpyTag-noro-VLP

Plasmid

#165989

Purpose

Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyTag-noro-VLP (ORF2 Synthetic, norovirus)

Use

Tags

SpyTag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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PL-SIN-PGK-EGFP

Plasmid

#21316

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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