159,134 results

1. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Orai1

   Plasmid

   #21638

   Depositor

   Anjana Rao

   Article

   Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

   Plasmid

   #208048

   Purpose

   Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   BirAopt
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   BirAopt is human-codon-optimized BirA (PMID 15707…

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. miReporter-CAG

   Plasmid

   #82478

   Purpose

   microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCD1A

   Plasmid

   #102952

   Purpose

   gene cloning of human CD1A

   Depositor

   Lucia Mori, Gennaro De Libero

   Article

   Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

   Insert

   CD1A cDNA (CD1A Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENN.AAV.CB7.CI.eGFP.WPRE.rBG

   Plasmid

   #105542

   Purpose

   AAV expression of EGFP from CB7 promoter

   Depositor

   James M. Wilson

   Has Service

   AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CB7

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET His6 NusA TEV LIC cloning vector (2N-T)

   Plasmid

   #29709

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-NusA-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Talin head-SpyCatcher003

   Plasmid

   #133566

   Purpose

   Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   EGFP-Talin head-SpyCatcher003
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

    Plasmid

    #182415

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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