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pCDNA3-SREBP-2-GFP

Plasmid

#204202

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

multiple silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-UCHL1

Plasmid

#22563

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL1 (UCHL1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

PLIN3 (PLIN3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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FRE5Cf

Plasmid

#62378

Purpose

Derived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DNAseI

Plasmid

#182408

Purpose

Bacterial expression of DNaseI

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DNase I (DNASE1 Bovine)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-WT

Plasmid

#25044

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pMK464 (mAB-Neo)

Plasmid

#214390

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BromoTag-Neo

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

Plasmid

#73796

Purpose

pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

Depositor

Ronald Davis

Article

Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

Inserts

dCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1, pRPR1(TetO), and pTef1

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-mouse Bcl6

Plasmid

#138410

Purpose

Express mouse Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (Bcl6 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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