We narrowed to 834 results for: Hr

1. pAG_P-HXT1-FAS1up

   Plasmid

   #126747

   Purpose

   vector for replacing the promoter of FAS1 coding gene

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   fas1 upstream
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   HXT1

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA[bTub+DsxF].1046A

   Plasmid

   #112694

   Purpose

   express two gRNA targeting bTub & DsxF under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-LoxP-PURO-LoxP-SNAP-TERT

   Plasmid

   #71390

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHSN501

   Plasmid

   #50589

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   zCas9D10A
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Cas9D10A nickase, was derived from the zCas9 and …

   Promoter

   2×35Sp and AtU6-26p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-LoxP-GFP-LoxP-SNAP-TERT

   Plasmid

   #71391

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-hrGFP II-TET1_CD

   Plasmid

   #83570

   Purpose

   Expresses the catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted

   Promoter

   CMV

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES-hrGFP II-mTET1_CD

   Plasmid

   #83571

   Purpose

   Expresses a mutant catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted, catalytic domain…

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. BlastR-P2A-DD-TCOF1-donor

   Plasmid

   #60748

   Purpose

   Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pUC57-white[coffee]

    Plasmid

    #84006

    Purpose

    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Insert

    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    A GC-to-AA mutation that creates a G589E missense…

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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