We narrowed to 1,414 results for: PUC57

1. pCP-tRNA

   Plasmid

   #133812

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJG100

   Plasmid

   #138966

   Purpose

   Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   wrmScarlet1-10
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Spike-VLP

   Plasmid

   #173922

   Purpose

   Expresses virus-like particles based upon the original SARS-Cov-2

   Depositor

   Andreas Stuermer

   Inserts

   SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
   SARS-Cov-2 E protein (E SARS-COV-2, Human, Synthetic)
   SARS-Cov-2 M Protein (M SARS-COV-2, Human, Synthetic)
   

   Use

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

   Available since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AICSDP-25:MAP1LC3B-mEGFP

   Plasmid

   #101783

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

   Depositor

   Allen Institute for Cell Science

   Insert

   MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSK-mU6-Cj-sgRNA-SV40-puro

   Plasmid

   #192128

   Purpose

   Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Cj-SgRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAP-AAV.FUS.3

   Plasmid

   #222333

   Purpose

   AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

   Depositor

   Jerzy Szablowski, Mikhail Shapiro

   Article

   Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

   Insert

   AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p41

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-64: FUS-mEGFP

   Plasmid

   #114410

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS

   Depositor

   Allen Institute for Cell Science

   Insert

   FUS Homology Arms with linker-mEGFP (FUS Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMGS54 (AID-P2A-Hygromycin)

   Plasmid

   #126583

   Purpose

   An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AID-P2A-HygR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNG1299

   Plasmid

   #149709

   Purpose

   Template for Malachite Green Aptamer (MGA) transcription assay

   Depositor

   Peter Lewis

   Article

   Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

   Insert

   Triple Promoter - 12X MG Aptamer Array - Terminator construct
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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