161,570 results

1. p23-NES-PspCas13b-msfGFP-NES-Flag

   Plasmid

   #165071

   Purpose

   overexpression of PspCas13b in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDZ2FE_Champ1_1-812

   Plasmid

   #105675

   Purpose

   Expresses Flag-tagged full-length human Champ1 protein

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAT-PB-CG2APtk

   Plasmid

   #86003

   Purpose

   homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   eGFP_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS2144

   Plasmid

   #104357

   Purpose

   AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

   Insert

   ssAAV-smCBA-EmGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   smCBA

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSP6-FR-2Strep

   Plasmid

   #210317

   Purpose

   Expresses SARS-CoV-2 NSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM378

   Plasmid

   #173174

   Purpose

   Expresses C9-1 gRNA-12xMBS from hU6 promoter

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   C9-1 targeting gRNA-12xMBS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. keratin 8 (krt8)-H2B-RFP

   Plasmid

   #105955

   Purpose

   transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   krt8 (KRT8 Human)
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CS2 Flag-Smad2 (3A)

   Plasmid

   #101763

   Purpose

   Expresses SMAD2 (3A) in mammalian cells

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   SMAD2 (SMAD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Three Ser to Ala mutations in the phosphorylation…

   Promoter

   CMV

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p23-NES-PguCas13b-msfGFP-NES-Flag

   Plasmid

   #165072

   Purpose

   overexpression of PguCas13b in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSSa30

    Plasmid

    #179502

    Purpose

    Tet-on red-shifted luciferase expression

    Depositor

    Dominique Sanglard

    Article

    Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

    Insert

    Red-shifted luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    TEF1t-HOP1 (p99CGH)

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. plex_307-ccdB-iRFP713

    Plasmid

    #201140

    Purpose

    pLEX_307 plasmid with iRFP713 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_GAS7-1/1

    Plasmid

    #91301

    Purpose

    Protein expression and purification of human SH3 domain construct GAS7-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GAS7-1/1 (GAS7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. R777-E189 Hs.PREX2

    Plasmid

    #70473

    Purpose

    Gateway ORF clone of human PREX2 [NM_024870.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PREX2 (PREX2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH41331

    Plasmid

    #47999

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pBS Olfactomedin1

    Plasmid

    #19225

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Olfactomedin 1 (OLFM1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP

    Plasmid

    #240124

    Purpose

    Express AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporation

    Depositor

    Abhishek Chatterjee

    Article

    Pham et al bioRxiv 2024.07.20.604406

    Inserts

    AAV2-ITR-EGFP
    MbPylRS
    PylOtR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-EFS-PCP-3xSNAP-NLS

    Plasmid

    #236388

    Purpose

    Overexpression of PCP-3xSNAP in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    PCP-3xSNAP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

    Plasmid

    #229812

    Purpose

    A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mCherry
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDCD1-DEM (P1#5)

    Plasmid

    #227738

    Purpose

    Designer Epigenome Modifier (DEM) designed to target the PDCD1-promoter

    Depositor

    Claudio Mussolino

    Article

    Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

    Insert

    Designer epigenome modifier targeting PDCD1-promoter
    

    Use

    TALEN

    Tags

    DNMT3A, DNMT3L, KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. LAG3-DEM (L3#1)

    Plasmid

    #227739

    Purpose

    Designer Epigenome Modifier (DEM) designed to target the LAG3-promoter

    Depositor

    Claudio Mussolino

    Article

    Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

    Insert

    Designer epigenome modifier targeting LAG3-promoter (Homo sapiens)
    

    Use

    TALEN

    Tags

    DNMT3A, DNMT3L, KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GreenT-EC - pRSET

    Plasmid

    #193947

    Purpose

    Bacterial expression vector for a low-affinity calcium indicator

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. plex_307-ccdB-EYFP

    Plasmid

    #201146

    Purpose

    pLEX_307 plasmid with EYFP marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT2/sh-Col1a1/GFP4_Seq1.2

    Plasmid

    #201403

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PC3-709-33-10_GAL4-FKBP12(F36V)

    Plasmid

    #200438

    Purpose

    Expresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target protein

    Depositor

    Xue Gao

    Article

    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

    Insert

    GAL4-FKBP12(F36V)
    

    Use

    Tags

    GAL4, FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FnR8-10

    Plasmid

    #182569

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179432

    Purpose

    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    HS4-TRE-cit-HS4-mch (DH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGWB14-LYX051

    Plasmid

    #126269

    Purpose

    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.

    Depositor

    Silin Zhong

    Insert

    Niben101Scf05690g01008.1
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFUSB4_AGGG

    Plasmid

    #48410

    Depositor

    Stephen Ekker

    Article

    Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

    Insert

    RVD sequence: NI NN NN NN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223-NTRK2

    Plasmid

    #23883

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK2 (NTRK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge