Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

159,258 results

Showing: 2431 - 2460 of 159258 results

  1. pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157027
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    JAG2 (JAG2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157027-plasmid-map-sequence-id-303163

    Enlarge

  4. Epac-S-H170

    Plasmid
    #170352
    Purpose
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170352-plasmid-map-sequence-id-345817

    Enlarge

  6. R777-E202 Hs.PRKAG2-nostop

    Plasmid
    #70486
    Purpose
    Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAG2 (PRKAG2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70486-plasmid-map-sequence-id-125935

    Enlarge

  8. pHOHO2_Hsp82viiA

    Plasmid
    #61708
    Purpose
    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.
    Depositor
    Dan Bolon
    Article
    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:
    Insert
    Hsp82viiA (HSP82 Budding Yeast)
    Use
    Yeast integration vector targeting inserts to the…
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    Heteromeric coiled coil inserted after amino acid…
    Promoter
    TEF1
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    61708-plasmid-map-sequence-id-104523

    Enlarge

  11. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

    Enlarge

  19. pBad-EBFP2

    Plasmid
    #14891
    Purpose
    Bacterial expression of EBFP2 blue fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Biochemistry. 2007 Apr 20. ():.
    Has Service
    Cloning Grade DNA
    Insert
    EBFP2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    14891-plasmid-map-sequence-id-457487

    Enlarge

  20. pFLAG_WT MDA5

    Plasmid
    #216798
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216798-plasmid-map-sequence-id-428831

    Enlarge

  24. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Article
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17870-plasmid-map-sequence-id-325133

    Enlarge

Showing: 2431 - 2460 of 159258 results