170,342 results

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pFasBac+GFP-CAMSAP2

Plasmid

#59037

Purpose

for baculovirus expression of CAMSAP2 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

Plasmid

#225752

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP

Plasmid

#225446

Purpose

Express mEGFP-fusion protein of interest

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pH-SpRY-PCE

Plasmid

#242428

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

pGSPE-epeg, SpRY-PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-FLAG

Plasmid

#205619

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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mScarlet-MYO10

Plasmid

#145179

Purpose

Expresses mScarlet-MYO10 in mammalian cells

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

mScarlet-MYO10 (MYO10 Human)

Expression

Mammalian

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pYFAC-ubi-Y-pyrG

Plasmid

#184497

Purpose

AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).

Depositor

Yit Heng Chooi

Article

Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus and …

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCHC042 (∆phaCAB)

Plasmid

#226208

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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hCas9_D10A

Plasmid

#41816

Purpose

Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9_D10A

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized, D10A nickase

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2-GAL4-UAS-Luc

Plasmid

#33020

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

6X GAL4-UAS

Tags

Luciferase

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

Plasmid

#207805

Purpose

E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

Depositor

Koen Breyne

Article

Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

Insert

E-NoMi

Use

Lentiviral

Tags

3xFlag tag, copGFP, mCherry, and nanoluciferase

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-hROSA26-1-1kbdonor

Plasmid

#234736

Purpose

PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

hROSA26-1 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG

Plasmid

#127299

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD_FLAG-GFP(Y39TAG)-6His

Plasmid

#223510

Purpose

Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Insert

GFP(Y39TAG)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

Y39TAG

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLX208-cytoFLARE1.0-TF

Plasmid

#234513

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLX208-cytoFLARE1.0-TEV

Plasmid

#234512

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (TEVp); the same TEVp involved in FLiCRE2

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

Lentiviral

Expression

Mammalian

Available Since

July 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mNeon-TurboID

Plasmid

#186610

Purpose

Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

mNeon-V5-TurboID

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

For reference see Branon et al., 2018

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Article

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Insert

MitoTimer

Expression

Mammalian

Mutation

F12S mutation in Timer

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-eGFP-Puro

Plasmid

#79823

Purpose

Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_MED13

Plasmid

#111689

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

MED13 (MED13 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGSPE-ccdb

Plasmid

#242424

Purpose

for pegRNA cloning

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

ccdb

Use

CRISPR

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP6-Myc-FLAG (puromycin)

Plasmid

#86851

Purpose

mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP6 (ATP6 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon corrected

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)

Plasmid

#40340

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

Blimp1 promoter (PRDM1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

Blimp1

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670nano

Plasmid

#127427

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano

Tags

His

Expression

Bacterial

Promoter

araC

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pmIL-6promoterEGFP

Plasmid

#112896

Purpose

To monitor the activation of the murine IL-6 promoter by eGFP expression

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

murine IL-6 promoter (Il6 Mouse)

Expression

Mammalian

Promoter

IL-6 promoter

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAG247 His-iFAST

Plasmid

#130808

Purpose

Expresses His-tagged iFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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