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Plasmid#230822PurposeAAV cloning vector for Flpo dependent expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only
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EF1a-Flex-MCS-WPRE-SV40 - GCH14
Plasmid#230821PurposeAAV cloning vector for CRE dependent expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV and Cre/Lox; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]
Plasmid#218344PurposeDonor plasmid of MAG Transposon with CD19 CAR mammalian expression cassetteDepositorInsertCD19 CAR mammalian expression cassette
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)
Plasmid#194231PurposeLentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB1-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
Plasmid#194232PurposeLentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB3-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)
Plasmid#194234PurposeLentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552 EF1a DIO Chronos GFP_Vgat gRNA
Plasmid#215277PurposeExpresses Vgat gRNA in a Cre dependent Chronos GFP vectorDepositorInsertVgat gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552 EF1a DIO Chronos GFP_VgatTTT gRNA
Plasmid#215278PurposeExpresses Vgat Control gRNA in a Cre dependent Chronos GFP vectorDepositorInsertVgat control gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP
Plasmid#216476Purposelentiviral dual expression of dsRed and tagBFPDepositorInsertsdsRed
tagBFP
UseLentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep
Plasmid#213542PurposeAAV viral vector packaging plasmid that expresses CoV2DepositorInsertCoV2 Orf3a-2xStrep (ORF3a Human, Severe acute respiratory syndrome coronavirus 2)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep
Plasmid#213543PurposeAAV viral vector packaging plasmid that expresses CoV2DepositorInsertCoV2 protE-2xStrep (E Human, Severe acute respiratory syndrome coronavirus 2)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211670PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-10 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211665PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-5 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211666PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-6 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only