We narrowed to 4,232 results for: pCMV

1. pCMV/3' Box_(GLuc)_TOP1

   Plasmid

   #68434

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-CD63-FKBP-Myc

   Plasmid

   #177910

   Purpose

   human CD63-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD63 (CD63 Human)
   

   Tags

   FKBP and Myc tag

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-XL4 ASXL1 (1-1304)

   Plasmid

   #74244

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-1304

   Promoter

   CMV

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCMVtrunc msfGFPΔC10-beta-actin

   Plasmid

   #231548

   Purpose

   Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-hAR-L26A,F27A

   Plasmid

   #89084

   Purpose

   mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR FxxLF mutant (AR Human)
   

   Expression

   Mammalian

   Mutation

   hAR-L26A,F27A

   Promoter

   CMV

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-myc-hIFITM3-Y20A

   Plasmid

   #58462

   Purpose

   Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Tyrosine 20 to alanine

   Promoter

   CMV IE

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-AID-Ns3h-UGI

   Plasmid

   #221219

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-Ns3h-UGI
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Flag-hMcl-1(S159A)

   Plasmid

   #25393

   Depositor

   Roger Davis

   Insert

   MCL1 (MCL1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 159 to Alanine

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-RosaR4 KKR mutations

   Plasmid

   #37199

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Insert

   FokI (fused to RosaR4 ZFN)
   

   Use

   Zfn expression

   Tags

   HA, ROSA26 eZFNs R4, and SV40 NLS

   Expression

   Mammalian

   Mutation

   I538K, E490K, and H537R

   Promoter

   CMV

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Tag2B EDD C2768A

    Plasmid

    #37189

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD C2768A (UBR5 Human)
    

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Changed Cysteine 2768 to Alanine

    Promoter

    CMV

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCas12a-ABE8e-V106W

    Plasmid

    #193646

    Purpose

    Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Expression

    Mammalian

    Mutation

    TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-OptoSTIM1(Cry2(D387A))

    Plasmid

    #70160

    Purpose

    Light-insensitive mutant of OptoSTIM1

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Flag-hMcl-1(T163A)

    Plasmid

    #25391

    Depositor

    Roger Davis

    Insert

    MCL1 (MCL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed threonine 163 to Alanine

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Neo-Bam E2F4

    Plasmid

    #37966

    Depositor

    Jacqueline Lees

    Article

    Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

    Insert

    E2F4 (E2F4 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-EE-UdgX-nCas9-UdgX

    Plasmid

    #163566

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SARS-CoV-2-Nsp3PLpro

    Plasmid

    #204208

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    PLpro of Nsp3

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Neo-Bam E2F6

    Plasmid

    #37965

    Depositor

    Jacqueline Lees

    Article

    Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

    Insert

    E2F6 (E2F6 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Neo-Bam E2F3b

    Plasmid

    #37975

    Depositor

    Jacqueline Lees

    Insert

    E2F3b (E2F3 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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