161,873 results

1. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL20_mEGFP_RUNX1_RUNX1T1

   Plasmid

   #205915

   Purpose

   Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   RUNX1_RUNX1T1 (RUNX1, RUNX1T1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7TGC

   Plasmid

   #24304

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT3GEPIR-CCND1shRNA2

   Plasmid

   #220578

   Purpose

   To inducibly knockdown CCND1 expression

   Depositor

   Sean Lee

   Article

   Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

   Insert

   CCND1 shRNA2 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TL024_lenti_hSyn:HaloTag-GluA1

   Plasmid

   #192517

   Purpose

   Expression of AMPA GluA1 with N terminal HaloTag fusion

   Depositor

   Adam Cohen

   Article

   Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

   Insert

   HaloTag-GluA1
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. minCMV-1xmNG-dMCP

   Plasmid

   #231091

   Purpose

   Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

   Depositor

   John Ngo

   Insert

   1xmNG-dMCP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-APPSwe-T2A-mCherry

    Plasmid

    #190804

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Article

    Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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