166,548 results

1. pcDNA3.1-GGamma3-GFP2

   Plasmid

   #166775

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma3-GFP2 (GNG3 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC-SV40NLS-Pfv-Sapphire

   Plasmid

   #203653

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. tol2-mpeg1-mcherry

   Plasmid

   #58935

   Purpose

   labels macrophages with mcherry

   Depositor

   Anna Huttenlocher

   Insert

   mpeg1-mcherry
   

   Promoter

   mpeg1

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-MMLVgag-3xNES-ABE8e-NG

   Plasmid

   #181754

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e-NG
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP FRNK

   Plasmid

   #50518

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

   Insert

   protein tyrosine kinase, mutated
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   truncated (NT2186-3268)

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. GSK3betaS9A  (1016)

   Plasmid

   #49492

   Purpose

   expresses human Gsk3beta with S9A (constitutively active) mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   GSK3beta S9A (GSK3B Human)
   

   Expression

   Mammalian

   Mutation

   S9A (constitutively active)

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Promoter

   cmv promoter

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-CD274-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156660

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CD274 (CD274 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH50900

   Plasmid

   #48047

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA-mito-GreenPy1Low

    Plasmid

    #239071

    Purpose

    Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Low
    

    Expression

    Mammalian

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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