164,577 results

1. CMVTO-Furin

   Plasmid

   #179920

   Purpose

   Furin protein

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Furin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH21-BNA-Luc

   Plasmid

   #187924

   Purpose

   Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymease I Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-JAK2

   Plasmid

   #23915

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRY2(L348F)-CreN

   Plasmid

   #75368

   Purpose

   Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Cre recombinase, residues 19-104

   Expression

   Mammalian

   Mutation

   L348F

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF CAG luc IRES neo

   Plasmid

   #98294

   Purpose

   For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Inserts

   Firefly luciferase
   IRES
   Neomycin resistance gene (NeoR)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIP-BSR-GFP11

    Plasmid

    #68716

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    bsr
    

    Use

    Retroviral

    Tags

    FLAG and GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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