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Plasmid#166416PurposeExpress pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)DepositorInsertcaenorhabditis elegans PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
Plasmid#166418PurposeExpress pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)DepositorInsertDrosophila melanogaster PRL (Prl Fly)
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)
Plasmid#166419PurposeExpress pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)DepositorInsertGaldieria sulphuraria PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)
Plasmid#166420PurposeExpress pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)DepositorInsertMonosiga brevicollis PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC_N168C His
Plasmid#173809PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationAsn168Cys for labelingPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM5
Plasmid#154166PurposeAedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM28
Plasmid#154165PurposeAedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virusDepositorInsertRel1A
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation16 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation12 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel2-specific…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterEnhancer for the Drosophila melanogaster Metchnik…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM51
Plasmid#154154PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB12_TtNAM-B1
Plasmid#124573PurposeExpresses full-length TtNAM-B1 with an N-terminal FLAG tag under the 35s promoter.DepositorInsertTaNAM-B1
UseTagsFLAGExpressionPlantMutationPromoter35sAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1
Plasmid#124575PurposeTtNAM-B1 CDS in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-B1
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TaNAM-A1(1-217)
Plasmid#124576PurposeNAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-A1 (1-217)
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1(1-215)
Plasmid#124577PurposeNAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.DepositorInsertTtNAM-B1 (1-215)
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H
Plasmid#108378PurposeFRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_WT-xrRNA-4H
Plasmid#108377PurposeFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
ReaLiSM A1-26link-GPIba+2cys
Plasmid#59580Purposeexpress VWF A1-GPIba ReaLiSM construct in mammalian cellsDepositorInsertVWF A1-linker-GPIba (VWF Human)
UseTags6HisExpressionMammalianMutationPromoterchick beta-actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only