We narrowed to 8,358 results for: BLI;
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Plasmid#120225PurposepCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.DepositorInsertSpCas9-NG
UseSynthetic BiologyTagsssrAExpressionMutationMutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…PromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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miR-124
Plasmid#182363PurposePlasmid with 4x miR-124 target sitesDepositorInsert4x miR-124 target sites
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD
Plasmid#117136PurposeEmpty vector encoding BFP-BSDDepositorInsertBSD2A
UseLentiviralTagsExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK651
Plasmid#71702PurposeProduces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)DepositorInsertthioredoxin reductase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
4MVT
Plasmid#60101PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPIAS3:YTC008-B10:C225910 (PIAS3 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103 FNBP3 WW domain #1
Plasmid#104233PurposeBacterial expression of WW domain #1 from FNBP3DepositorInsertFNBP3 WW domain #1 (PRPF40A Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-H2B-sfGFP-T2A-iCre
Plasmid#183351PurposeHistone H2B-sfGFP and iCre recombinaseDepositorInsertHistone H2B-sfGFP-T2A-iCre
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p221-RV-dlx-dio-Tdtomato
Plasmid#185697PurposeRetroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoterDepositorInsertmembrane Tdtomato
UseCre/Lox and RetroviralTagsGap43 palmitoylationExpressionMutationPromotermDlxAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCP-GPI Control Plasmid
Plasmid#101132PurposeExpression of MCP-Tagged GPI anchor in Mammalian cellsDepositorInsertGPI
UseTagsMCP-tag (Mutant ACP- tag)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUS252b
Plasmid#191831PurposeExpresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbonesDepositorInsertfuGFPb
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos3.2-Vinculin-N-21
Plasmid#66952PurposeLocalization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580DepositorInsertVinculin (VCL Human)
UseTagsmEos3.2ExpressionMammalianMutationV234L in VinculinPromoterCMVAvailable sinceJuly 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-S-B.1.617.2-FLAG
Plasmid#177097PurposeB.1.617.2 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein B.1.617.2 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135978PurposegRNA against the right loxP site of the Ai9 alleleDepositorInsertAi9 gRNA2
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterCBAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-C840W/F869D/F979A/N980A
Plasmid#28144DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationC840W, F869D, F979A, N980APromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135979PurposegRNA against the left loxP site of the Ai9 alleleDepositorInsertAi9 gRNA1
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX381 Filifactor alocis Cas9
Plasmid#68318PurposeFilifactor alocis Cas9DepositorInsertFaCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB Neo* attP
Plasmid#138412PurposeattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Att/phic31TagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK217
Plasmid#72471PurposeProduces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-alphaMHC 3300bp
Plasmid#51934Purposeluciferase reporter for rat alpha-MHC promoterDepositorInsertalpha MHC (Myh6 Rat)
UseLuciferaseTagsluciferaseExpressionMutation-2930 to +410 promoter regionPromoteralpha MHCAvailable sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-TVA66T-EGFP
Plasmid#64091PurposeExpresses TVA66T-EGFP under the CAG promotorDepositorInsertTVA66T-EGFP
UseAAV and Cre/LoxTagsEGFPExpressionMammalianMutationGlutamic acid 66 to ThreoninePromoterCAGAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX385 Streptococcus pasteurianus Cas9
Plasmid#68322PurposeStreptococcus pasteurianus Cas9DepositorInsertSpaCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Elavl2IR
Plasmid#120518PurposedsRNA expressionDepositorInsertElavl2 (Elavl2 Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACP-GPI Control Plasmid
Plasmid#101131PurposeExpression of ACP-Tagged GPI anchor in Mammalian cellsDepositorInsertGPI
UseTagsACP-Tag (ACP wt)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
Plasmid#45779DepositorInsertsigma factor 28
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP
Plasmid#178433PurposeCAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)DepositorInserttd-sfGFP
UseCre/Lox; Flp/frt, piggybacTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/TR
Plasmid#110358PurposeMammalian retroviral expression of Tetracycline repressor with β-globin intron for doxycycline inducible systemsDepositorInsertTetracycline repressor
UseRetroviralTagsExpressionMammalianMutationPromotergag truncatedAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Cep215-FL
Plasmid#106911PurposeMammalian expression of CEP215DepositorInsertCEP215 (Cdk5rap2 Mouse)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-hASIC1
Plasmid#32790DepositorInsertASIC1 (ASIC1 Human)
UseCloningTagsExpressionMutationPromoterplacAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP2
Plasmid#78120PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP2
UseTagsKDEL and calreticulin signal peptideExpressionMammalianMutationD24N/D119A in aequorin moietyPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)
Plasmid#48291DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-AFB2
Plasmid#99531PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorInsertAFB2 (AFB2 Mustard Weed)
UseTagsExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUS252s
Plasmid#191833PurposeExpresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbonesDepositorInsertfuGFPs
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSV40-GUS-P2A-Hygro
Plasmid#106480PurposeExpression of GUS and Hygromycin resistance gene from SV40 promoterDepositorInsertsbeta-glucuronidase
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_TetOn_Hu_MAD2(WT)
Plasmid#136347PurposeLentiviral inducible expression (TetOn) of human MAD2DepositorInsertMAD2L1 (MAD2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHuR CDS
Plasmid#110426PurposeTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.DepositorInsertELAVL1 HuR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -