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pRRG581-FLI1

Plasmid

#99099

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea FLI-1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

FLI-1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG377-CNG1

Plasmid

#99098

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea CNG1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

CNG1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG882-F-spondin

Plasmid

#99096

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea F-spondin

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

F-spondin in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG1014-LDLRR-3

Plasmid

#99095

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-3

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

LDLRR-3 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG170-LDLRR-2

Plasmid

#99094

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-2

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

LDLRR-2 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG1013-LDLRR-1

Plasmid

#99093

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

LDLRR-1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG101-mblk

Plasmid

#99091

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea mblk

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

mblk in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG796-jagged-like

Plasmid

#99103

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea jagged-like

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

jagged-like in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG763-CRELD

Plasmid

#99092

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea CRELD

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

CRELD in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R(1-300)

Plasmid

#79029

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5-21R (1-300)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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p3.2mar-Luc

Plasmid

#63696

Purpose

3.2 kb marine armor plate enhancer driving Luciferase expression

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Tags

Luciferase

Expression

Mammalian

Promoter

TATA box

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-mTurquoise2-Nuf2

Plasmid

#80762

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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35S-VP16-GWY/pAM

Plasmid

#201234

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-VP16-GWY

Expression

Plant

Promoter

CaMV 35S

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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35S-GWY-VP16/pAM

Plasmid

#201232

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-VP16

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (mCherry)

Plasmid

#102945

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only
PresentER-SIINFEKL (GFP)

Plasmid

#102944

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-iRFP720-E2A-Luc

Plasmid

#104587

Purpose

Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

Depositor

Antonius Plagge

Article

Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

Insert

iRFP720-E2A-Luciferase

Use

Lentiviral and Luciferase

Tags

bi-cistronic

Expression

Mammalian

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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