We narrowed to 13,043 results for: SHI

1. pIRESpuroN3xFLAG-UFSP2

   Plasmid

   #204553

   Purpose

   Expresses 3xFLAG-tagged UFSP2 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFSP2 (UFSP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRESpuroN3xFLAG-His-CDK5RAP3

   Plasmid

   #204555

   Purpose

   Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLR5-CBh-dCas9-hUtx-IRES-Hyg

   Plasmid

   #122374

   Purpose

   Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   CBh-dCas9-hUtx-IRES-Hyg-pA
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C1-hAtg13

   Plasmid

   #22875

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. POLR2A-N CRISPR pX330

   Plasmid

   #124495

   Purpose

   A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

   Depositor

   Masato Kanemaki

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   POLR2A targeting CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG-CMV10-hAtg13

   Plasmid

   #22872

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pET48 AtGUN1-PS

   Plasmid

   #136358

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLR5-CBh-dCas9-hEzh2-IRES-Hyg

    Plasmid

    #122375

    Purpose

    Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    CBh-dCas9-hEzh2-IRES-Hyg-pA
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDicAID_nCas9-PmCDA-2A-NptII_ETR

    Plasmid

    #91695

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIRESpuroN3xFLAG-UFSP2 C302A

    Plasmid

    #204554

    Purpose

    Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFSP2 (UFSP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C302A

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET48 AtGUN1-SMR1

    Plasmid

    #136361

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET48 AtGUN1-SMR2

    Plasmid

    #136362

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET48 AtGUN1-PPR1

    Plasmid

    #136359

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET48 AtGUN1-PPR2

    Plasmid

    #136360

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6p-1-OSF-UFM1

    Plasmid

    #204565

    Purpose

    Expresses GST-Strep-FLAG-tagged UFM1 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFM1 (UFM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    delta84-85aa

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px335-EF-Slc2a3-L

    Plasmid

    #122304

    Purpose

    Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only