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Showing: 1641 - 1660 of 15693 results
  1. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  2. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

    Plasmid
    #115788
    Purpose
    Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.
    Depositor
    Insert
    LRP5 (LRP5 Human)
    Tags
    3xflag
    Expression
    Mammalian
    Mutation
    contains silent mutation that creates an internal…
    Promoter
    CMV
    Available since
    Oct. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pEGFP-Rab21-Q76L (CA)

    Plasmid
    #83422
    Purpose
    Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Insert
    Rab21 (Rab21 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residue 76 from Q to L (Q76L: CAG -> CTG)
    Promoter
    CMV
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1641 - 1660 of 15693 results