We narrowed to 76,952 results for: rest

1. pOpen-SfiIR (cDNA)

   Plasmid

   #165528

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   SfiI (used to create cDNA libraries)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLenti-sgRNA

   Plasmid

   #71409

   Purpose

   There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

   Depositor

   David Sabatini, Eric Lander

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

   Plasmid

   #139015

   Purpose

   To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   HA-MAO

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-APEX3-C Cloning Vector

   Plasmid

   #220615

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   Flag-APEX3 fusion

   Expression

   Mammalian

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLfa[Tc'hsp5'3'UTR]fa

   Plasmid

   #86450

   Purpose

   Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)

   Depositor

   Gregor Bucher

   Insert

   Tc'hsp5'3'UTR
   

   Expression

   Insect

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-2xAPEX2-C Cloning Vector

   Plasmid

   #136991

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEM

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 371-U

   Plasmid

   #97036

   Purpose

   MYFQSNA-yORF

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Tags

   MYFQSNA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-Spe4-Kozak-miRFP713-RfA

   Plasmid

   #186381

   Purpose

   Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-Spe4-RfA-miRFP713-stop

   Plasmid

   #186391

   Purpose

   Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.3G_X7

    Plasmid

    #171213

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites

    Depositor

    Jenny Wang

    Insert

    N/A; empty backbone
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSNAP-tag (T7) Vector

    Plasmid

    #101137

    Purpose

    Expression of protein of interest with SNAP-Tag in bacterial cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Tags

    SNAP-tag (SNAP26b)

    Expression

    Bacterial

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid

    #72299

    Purpose

    Express gene of interest under CB promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    chicken beta-actin + CMV enhancer

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDEST-Spe4-RfA-mScarlet-I-stop

    Plasmid

    #186393

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-Spe4-RfA-Halotag-stop

    Plasmid

    #186387

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-Spe4-Kozak-Snaptag-RfA

    Plasmid

    #186385

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-Spe4-RfA-emiRFP703-stop

    Plasmid

    #186386

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid

    #186376

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-Spe4-RfA-mVenus-stop

    Plasmid

    #186394

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-Spe4-Kozak-Halotag-RfA

    Plasmid

    #186377

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-Spe4-RfA-Snaptag-stop

    Plasmid

    #186395

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge