161,873 results

1. pEGFP-parkin C431S

   Plasmid

   #45877

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C431S mutation (ubiquitin acceptor site)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

   Plasmid

   #199781

   Purpose

   Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

   Depositor

   Sascha Martens

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   GST-Thrombin-TEV-EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP_sfCherry2(11)_HP1

   Plasmid

   #82607

   Purpose

   Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_HP1b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pGSTag

   Plasmid

   #21877

   Depositor

   David Ron

   Article

   Ron et al Biotechniques. 1992 Dec . 13(6):866-9.

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. sox10:mCherry

   Plasmid

   #98695

   Purpose

   I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

   Depositor

   Thomas Look

   Article

   Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

   Insert

   mCherry
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP71-tCD19-mGFP

   Plasmid

   #174609

   Purpose

   expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   CD19 extracellular and transmembrane domain (Cd19 Mouse)
   

   Use

   Tags

   palmitoylated EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-fDIO-pAce-Kv2.1PR

   Plasmid

   #195535

   Purpose

   Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CAG

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2_mSTING_gRNA_1

   Plasmid

   #196625

   Purpose

   Knock-out STING in murine cells, gRNA 1.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING gRNA 1 (Sting1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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