166,289 results

1. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

   Plasmid

   #219565

   Purpose

   3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   HygroR_2A_H2B-GFP-N_loxP_mScarlet
   

   Use

   Unspecified

   Promoter

   EF-1a

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-CCND1

   Plasmid

   #116721

   Purpose

   Lentiviral expression of CCND1

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAD-F3-NPEST-iCRE-2A-mCherryPEST

   Plasmid

   #99608

   Purpose

   Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   iCRE
   mCherry
   

   Use

   Unspecified

   Tags

   PEST

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 768 pME mApple-CAAX

   Plasmid

   #195961

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple-CAAX (membrane)
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET32a-hHBEGF

   Plasmid

   #199234

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proheparin-binding EGF-like growth factor (HBEGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

   Plasmid

   #107137

   Purpose

   Dominant negative PKA cGAL effector for C. elegans

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   kin-2a(G310D)::SL2::GFP
   

   Expression

   Worm

   Promoter

   15xUAS-delta-pes-10(basal promoter)

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1A-CD19.8h.8TM.28.3z

   Plasmid

   #200670

   Purpose

   Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR103

   Plasmid

   #187242

   Purpose

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
   

   Use

   Lentiviral

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK123 pDESTattB_exorh:mCerulean

   Plasmid

   #213512

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only