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Showing: 2491 - 2520 of 74675 results

  2. psiCHECK2-let-7 WT

    Plasmid
    #78260
    Purpose
    psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    fully complimentary let-7 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78260-plasmid-map-sequence-id-149453

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  4. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  6. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78261-plasmid-map-sequence-id-149478

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  8. p5E-mfap4

    Plasmid
    #70052
    Purpose
    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.
    Depositor
    David Tobin
    Article
    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do
    Insert
    mfap4 promoter
    Use
    Gateway 5' element
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70052-plasmid-map-sequence-id-130081

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  12. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    4-μHOM variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

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  16. pLenti-TetO-PM

    Plasmid
    #197387
    Purpose
    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
    Depositor
    Qiao Zhou
    Article
    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038
    Insert
    PDX1-T2A-MAFA
    Use
    Lentiviral; Teto
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    197387-plasmid-map-sequence-id-385579

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  17. PB-CAG::Nucbow

    Plasmid
    #158996
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Nucbow
    Use
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158996-plasmid-map-sequence-id-311805

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  19. pLyGo-Ec-8

    Plasmid
    #163136
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P(rhaB)
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163136-plasmid-map-sequence-id-332138

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  22. pCLucIPZ

    Plasmid
    #53222
    Purpose
    Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest
    Depositor
    Thorsten Stiewe
    Article
    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1
    Inserts
    Cypridina luciferase
    non-silencing shRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53222-plasmid-map-sequence-id-342548

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  29. pCAP01

    Plasmid
    #59981
    Purpose
    The function of this material is to facilitate the capture and expression of bacterial gene clusters.
    Depositor
    Bradley Moore
    Article
    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111
    Type
    Empty backbone
    Use
    Synthetic Biology; Transformation-associated reco…
    Tags
    no
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    no
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59981-plasmid-map-sequence-id-96247

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Showing: 2491 - 2520 of 74675 results