We narrowed to 24,628 results for: Nes

1. pMaCTag-29

   Plasmid

   #120008

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMiniwhiteA1

   Plasmid

   #165872

   Purpose

   Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Eye Marker Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMaCTag-04

   Plasmid

   #119983

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mTFP1
   

   Use

   Pcr template

   Tags

   mTFP1

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. OeNL-Actin_pcDNA3

   Plasmid

   #89524

   Purpose

   Orange color variant of bright luminescent protein, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS316Nab3

   Plasmid

   #110533

   Purpose

   Expresses yeast NAB3 using the endogenous promoter

   Depositor

   Daniel Reines

   Article

   Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

   Insert

   NAB3 (NAB3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSR270

   Plasmid

   #14057

   Depositor

   Richard Hynes

   Article

   Schwarzbauer et al Cell. 1983 Dec . 35(2 Pt 1):421-31.

   Insert

   Rat Fibronectin (Fn1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pBS-tWnt5a

   Plasmid

   #169372

   Purpose

   Expresses a part of Chinese soft-shell turtle Wnt5a for in situ hybridization probe.

   Depositor

   Katsuhiko Ono

   Article

   Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

   Insert

   Wnt5a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-tWnt7b

   Plasmid

   #169371

   Purpose

   Expresses a part of Chinese soft-shell turtle Wnt7b for in situ hybridization probe.

   Depositor

   Katsuhiko Ono

   Article

   Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

   Insert

   Wnt7b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMaCTag-08

   Plasmid

   #119987

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mClover3
   

   Use

   Pcr template

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGB1_alpha1R

    Plasmid

    #68226

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGB1_alpha2R

    Plasmid

    #68227

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AlphaE

    Plasmid

    #124527

    Purpose

    pColE1_AlphaE

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaCTag-14

    Plasmid

    #119993

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeptune2.5
    

    Use

    Pcr template

    Tags

    mNeptune2.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaCTag-24

    Plasmid

    #120003

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TAP
    

    Use

    Pcr template

    Tags

    TAP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-10

    Plasmid

    #119989

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKOk
    

    Use

    Pcr template

    Tags

    mKOk

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114105

    Purpose

    Cre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMaCTag-33

    Plasmid

    #119986

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    FLAG
    

    Use

    Pcr template

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry pBSDONR P4r-P2

    Plasmid

    #121528

    Purpose

    For making C-terminal mCherry fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_dCas9-1x5

    Plasmid

    #63599

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST HP1 gamma deltaC

    Plasmid

    #24077

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Missing the C-terminal 25 amino acids

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pX330A_dCas9-1x3

    Plasmid

    #63597

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KIF1C-FHA

    Plasmid

    #25549

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KIF1C-FHA (KIF1C Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    FHA domain only

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pFLIP30

    Plasmid

    #65499

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-mCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFLIP32

    Plasmid

    #65500

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFLIP39

    Plasmid

    #65507

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLSLZ.D

    Plasmid

    #51496

    Purpose

    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. U371-GFP ILtoEK

    Plasmid

    #24472

    Depositor

    Ron Vale

    Article

    Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

    Insert

    Unc104 (unc-104 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Contains only the kinesin's motor head fused…

    Promoter

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pccGFP-MalE-pos-2H01

    Plasmid

    #239760

    Purpose

    Positive control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of SEM5A
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Leucine 985 to Isoleucine

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pccGFP-MalE-neg-2H11

    Plasmid

    #239086

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of EVA1B
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Leucine 30 to Isoleucine

    Promoter

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pccGFP-MalE-pos-1F02

    Plasmid

    #239087

    Purpose

    Positive control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of AMPE
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    deleted amino acids 19-22, and changed Leucine 35…

    Promoter

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pccGFP-MalE-pos-2H07

    Plasmid

    #239088

    Purpose

    Positive control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of FND10
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Valine 184 to Isoleucine

    Promoter

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pccGFP-MalE-neg-2E11

    Plasmid

    #239084

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of LRIG3
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Valine 827 to Isoleucine

    Promoter

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pccGFP-MalE-neg-2F12

    Plasmid

    #239085

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of LRIG3
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Valine 815 to Isoleucine

    Promoter

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBAD-luxNeon

    Plasmid

    #229942

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxNeon
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. JD73

    Plasmid

    #229482

    Purpose

    Adenoviral VA RNA I & II genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    VA RNA I & II
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEC2796 (pSpy0C4)

    Plasmid

    #220280

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMC-1-ZmUbi1-6

    Plasmid

    #197714

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmUbi1 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMC-1-PvUbi1-6

    Plasmid

    #197705

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    PvUbi1 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCEP13

    Plasmid

    #207584

    Purpose

    TolQR expression vector for purification (TolQ-TolR-TEV-Strep_His)

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    TolQ-TolR-TEV-Strep_His
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJS110

    Plasmid

    #207591

    Purpose

    GFP-AIA H22A expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-AIA H22A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge