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Showing: 4151 - 4200 of 5577 results

  1. pFastBacHTa_HCK

    Plasmid
    #224245
    Purpose
    Expression of human HCK Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Tags
    6xHis-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224245-plasmid-map-sequence-id-458377

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  2. pFastBacHTa_LYN

    Plasmid
    #224244
    Purpose
    Expression of human LYN Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Tags
    6xHis-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224244-plasmid-map-sequence-id-458379

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  10. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

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  12. Lmx1a-in situ probe

    Plasmid
    #45071
    Depositor
    Kathleen Millen
    Insert
    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    Expression
    Mammalian
    Mutation
    Lmx1a residues 94-1149
    Available Since
    May 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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  23. pOpen-Endonuclease VIII E. coli

    Plasmid
    #165561
    Purpose
    Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease VIII E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165561-plasmid-map-sequence-id-339954

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  24. pMX-STAT5A-DN(-351)-Neo

    Plasmid
    #83249
    Purpose
    Retroviral expression of STAT5A dominant negative C-terminal deletion
    Depositor
    Andrew Brooks
    Insert
    Stat5A (Stat5a Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    C-terminal deletion of 351 nucleotides (dominant …
    Available Since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83249-plasmid-map-sequence-id-173238

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  26. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  29. pNtCp-rbcL-PHLS-aadA-accD

    Plasmid
    #52470
    Purpose
    Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-aadA-accD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52470-plasmid-map-sequence-id-81333

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  31. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

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  35. pJF16

    Plasmid
    #253747
    Purpose
    Encodes GFP_AID* and pALD6-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    ALD6 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253747-plasmid-map-sequence-id-507847

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  36. pJF15

    Plasmid
    #253746
    Purpose
    Encodes GFP_AID* and pRPL18B-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    RPL18B and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253746-plasmid-map-sequence-id-507840

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  37. pJF17

    Plasmid
    #253748
    Purpose
    Encodes GFP_AID* and pRNR2-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    RNR2 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253748-plasmid-map-sequence-id-507839

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  38. pJF18

    Plasmid
    #253749
    Purpose
    Encodes GFP_AID* and pREV1-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    REV1 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253749-plasmid-map-sequence-id-507834

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  39. pJF19

    Plasmid
    #253750
    Purpose
    Encodes GFP_AID* and pSAC6-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    SAC6 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253750-plasmid-map-sequence-id-507833

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  41. Tet-O-FUW-NR2F6-PURO

    Plasmid
    #251733
    Purpose
    Overexpression construct expressing NR2F6-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    NR2F6 (NR2F6 Human)
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251733-plasmid-map-sequence-id-501477

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  42. pSELIS-SRTF1

    Plasmid
    #246681
    Purpose
    Used for growth-based enrichment of functional transcription factor variants and fluorescent-based readout of transcription factor function.
    Depositor
    Andrew Ellington
    Inserts
    SRTF1-responsive promoter
    SRTF1-responsive promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    246681-plasmid-map-sequence-id-493947

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  49. pAGW-Fray

    Plasmid
    #203979
    Purpose
    GFP-Fray under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Tags
    EGFP
    Expression
    Insect
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203979-plasmid-map-sequence-id-403220

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  50. pAMW-WnkAAxV

    Plasmid
    #203980
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    RFxV mutated to AAxV
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203980-plasmid-map-sequence-id-403221

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Showing: 4151 - 4200 of 5577 results