We narrowed to 2,220 results for: SE

1. MEK1 (S218D,S222D)-pcw107

   Plasmid

   #64604

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S218D, S222D

   Promoter

   PGK

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. SmoM2 (W535L)-pcw107-V5

   Plasmid

   #64628

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   W535L

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3-OGDH_wobble

   Plasmid

   #110938

   Purpose

   OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   OGDH wobble cDNA (OGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   15 silent wobble mutations corresponding to three…

   Promoter

   SV40

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgCXCR4-2

   Plasmid

   #46917

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgCXCR4 -2 (CXCR4 Human)
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. beta-catenin (S33Y)-pcw107

   Plasmid

   #64615

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S33Y

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgCD71-2

   Plasmid

   #46918

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgCD71 -2 (TFRC Human)
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SmoM2 (W535L)-pcw107

   Plasmid

   #64627

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   W535L

   Promoter

   PGK

   Available Since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-hAR-H874Y

   Plasmid

   #89087

   Purpose

   mammalian expression of human androgen receptor with prostate cancer somatic mutation H874Y from CWR22 xenograft

   Depositor

   Elizabeth Wilson

   Article

   Tan et al Mol Endocrinol. 1997 Apr;11(4):450-9. do

   Insert

   hAR-H874Y prostate cancer mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-H874Y

   Promoter

   CMV

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pET32bplus_trxAChEwt

    Plasmid

    #83916

    Purpose

    Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

    Depositor

    Sarel Fleishman

    Article

    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

    Insert

    Short form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    

    Use

    Tags

    His, S-tag, and TRX

    Expression

    Bacterial

    Mutation

    codon optimised for E.coli expression

    Promoter

    T7 promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1408

    Plasmid

    #29205

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-Tet-O-Sox5-Puro

    Plasmid

    #221667

    Purpose

    Expresses Sox5 in mammalian cells

    Depositor

    Vanessa Hall

    Article

    Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

    Insert

    Sox5 (Sox5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Notch3 intracellular domain-pcw107-V5

    Plasmid

    #64623

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Notch1 intracellular domain-pcw107-V5

    Plasmid

    #64622

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pMP71-hPDL-1

    Plasmid

    #118631

    Purpose

    human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    hPDL-1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ad:2CMV_A2R2-N25K3

    Plasmid

    #122242

    Purpose

    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-Tet-On-Arl13b-RhoA sensor

    Plasmid

    #189613

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Arl13b
    

    Use

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Tet3G

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. beta-catenin (S33Y)-pcw107-V5

    Plasmid

    #64616

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S33Y

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. TCF4-DN in pLX303

    Plasmid

    #42592

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    31 first amino acids of hTcf4 have been deleted

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pGAPTrap-mtagBFP2-IRESMuro

    Plasmid

    #82335

    Purpose

    Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagBFP2-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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