We narrowed to 24,400 results for: lars
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Plasmid#213417PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A-E1021A
Plasmid#213414PurposeVinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcS-RDG-C1C2
Plasmid#200163PurposeThis plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsRDG-C1C2
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 hU6 ACTB sgRNA
Plasmid#206270PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNADepositorInsertACTB sgRNA
UseCRISPR; Multimate/gateway entr 2TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 hU6 HEK3 PegRNA
Plasmid#206277PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNADepositorInsertHEK3 PegRNA
UseCRISPR; Multimate/gateway entr 2TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 polH aeBlue polH VSV-G
Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3TagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(T304I)
Plasmid#177133PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)DepositorInsertPolB (POLB Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K72A)
Plasmid#177132PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)DepositorInsertPolB (POLB Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolB(T304I)-Puro
Plasmid#177143PurposeLentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K206A/K244A)
Plasmid#177135PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)DepositorInsertPolB
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-SspB-HaloTag-TIAM1-DHPH
Plasmid#176134PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Lrig1-T2A-tdTomato-FNF-TK
Plasmid#134320PurposeB6J Lrig1-T2A-tdTomato allele targeting vector, low copy numberDepositorInsertLrig1 (Lrig1 Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tdTomatoExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB6-Cdk6-T2A-td-sfGFP-FNF-TK
Plasmid#134321PurposeB6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy numberDepositorInsertCdk6 (Cdk6 Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tandem dimer-superfolder…ExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3/gamma2SIL
Plasmid#121433PurposeGABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1/gamma2SIL
Plasmid#121432PurposeGABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
YCe2741 HC_Kan_EF1ap_p18
Plasmid#100698Purposepart designed to occupy position 18 of EMMA. Functional category: Constitutional promoterDepositorInsertEF1a promoter (EEF1A1 Human)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-FYN-N-10
Plasmid#55483PurposeLocalization: Membrane Associated Kinase, Excitation: 462, Emission: 492DepositorInsertFYN (FYN Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cilantro 2
Plasmid#74450PurposeCan clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-VP64-BFP
Plasmid#46912PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFPDepositorInsertsdCas9-VP64-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags3xNLS, BFP, and VP64 domainExpressionMammalianMutationPromoterLTR and PGKAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only