We narrowed to 5,538 results for: pcas

1. pCAG-MBP-ATG9-P834A

   Plasmid

   #188082

   Purpose

   Expresses human ATG9-P834A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Use

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   P834A

   Promoter

   CMV

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gma-pCAMBIA-1301-STTM171

   Plasmid

   #84080

   Purpose

   Disturb the expression of miR171 in soybean

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR171
   

   Use

   Tags

   Expression

   Bacterial, Plant, and Yeast…

   Mutation

   Promoter

   2x35S

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-GFP-PQRv3-RFP

   Plasmid

   #73951

   Purpose

   Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

   Depositor

   Brian Chen

   Article

   Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

   Inserts

   sfGFP (superfolder GFP)
   RFP
   

   Use

   Tags

   A residual PQR peptide wii be left at the C-termi…

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin and Chicken beta actin (shared…

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GBP1-10gly-lexADBD

   Plasmid

   #50795

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP1-lexA DNA binding domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-attBr-ZsGreen-attP

   Plasmid

   #51265

   Purpose

   Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-ZsGreen-attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-EGFP/RFP-int

   Plasmid

   #19818

   Depositor

   Zuoshang Xu

   Article

   Qiu et al BMC Biotechnol. 2008 . 8():77.

   Type

   Empty backbone

   Use

   Cre/Lox and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-Bgh-GFP-Farpl

   Plasmid

   #115501

   Purpose

   expression of rat Farp1

   Depositor

   Thomas Biederer

   Article

   Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

   Insert

   Farp1 (Farp1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin

   Available since

   Dec. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R, R883K, R887A

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid

    #159363

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

    Depositor

    Michael Farzan, Hyeryun Choe

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag and Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Michael Farzan, Hyeryun Choe

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

    Plasmid

    #196197

    Purpose

    Cas9 + gRNA expression to edit G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-Flag-hsDicer (E1564A)

    Plasmid

    #41587

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E1564A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCaSpeR4 mito roGFP2-Grx1

    Plasmid

    #65000

    Purpose

    Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCASP-SptP120-3G124-HilA

    Plasmid

    #153323

    Purpose

    Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3G124 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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