We narrowed to 13,284 results for: sequence

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pLBPVXBa-M

Plasmid

#229079

Purpose

New version of a PVX vector to express heterologous sequences in plants

Depositor

Jose-Antonio Daros

Article

Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

Insert

Potato virus X

Expression

Plant

Mutation

Mutation coat protein start codon (ATG to AGG); d…

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-Vn

Plasmid

#183153

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

Plasmid

#70191

Purpose

Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

ClpX (clpX )

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-noPS

Plasmid

#177943

Purpose

Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP

Expression

Mammalian

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEarleyGate 100RMOA mPing

Plasmid

#196137

Purpose

Measures mPing transposition in plants

Depositor

Charles Nathan Hancock

Article

Renken et al MicroPubl Biol. 2023 May 16;2023:10.1791

Inserts

Pong TPase LA T2A ORF1SC1 ONE
mPing

Use

Binary

Expression

Plant

Mutation

TPase NES sequence mutated L418A, L420A; T2A pept…

Promoter

35S and RPS5a

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pYL322d1/Vn-C

Plasmid

#183154

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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BB3cH_pGAP_23*_pPFK300_Cas9

Plasmid

#104912

Purpose

hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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