We narrowed to 4,867 results for: gne

1. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSCALPs-HA-NFAT1 (4-460)-GFP

   Plasmid

   #88879

   Purpose

   Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP fusion protein and HA tag

   Expression

   Mammalian

   Mutation

   amino acids 4-460 only; L78P & L287P *see bel…

   Promoter

   SFFV (spleen focus forming virus)

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. F40 tension sensor

   Plasmid

   #101252

   Purpose

   Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-F40-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. QUAS-jGCaMP7s-T2A-tdTomato

   Plasmid

   #200010

   Purpose

   QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   3xP3-ECFP-jGCaMP7s-td-Tomato
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ucp1-empty-miR122

   Plasmid

   #231695

   Purpose

   Empty control vector for pAAV-Ucp1-Cre-miR122

   Depositor

   Joerg Heeren

   Article

   Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   rat Ucp1 enhancer-promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SR140

   Plasmid

   #156037

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SR140 (U2SURP Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. THOC1

   Plasmid

   #156081

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   THOC1 (THOC1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBRPyCAG-hKlf2-DsRed-Ip

   Plasmid

   #26277

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

   Insert

   Klf2 (KLF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAR-B1(1986.1)

    Plasmid

    #78503

    Purpose

    Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid encodes AAV2 Rep and AAV-B1 capsid

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV-B1 capsid
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW17 - pAdapter

    Plasmid

    #174664

    Purpose

    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Insert

    MS2 coat protein
    

    Use

    Tags

    λCI

    Expression

    Bacterial

    Mutation

    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

    Promoter

    Synthetic Constitutive Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis6-hDF-deltaTM-pCMV-9

    Plasmid

    #72587

    Purpose

    human dysferlin no transmembrane domain tagged with his tag

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    transmembrane domain deleted

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-nLight3.1

    Plasmid

    #111061

    Purpose

    Expresses nLight3.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    nLight3.1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. stabilized HP35 tension sensor module

    Plasmid

    #101251

    Purpose

    Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-HP35st-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. UAS-u(S)Cas9

    Plasmid

    #127383

    Purpose

    Expresses Cas9 at high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(S)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ145

    Plasmid

    #98405

    Purpose

    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SYMPK

    Plasmid

    #156071

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SYMPK (SYMPK Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYZ149

    Plasmid

    #98407

    Purpose

    Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
    downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge