Addgene

We narrowed to 5,877 results for: pcDNA 3.1

Showing: 2521 - 2550 of 5877 results

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pcDNA3-ING2

Plasmid

#13294

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-Mirk (YF)

Plasmid

#17845

Depositor

Eileen Friedman

Article

Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Insert

Mirk (DYRK1B Human)

Use

Tags

6xHis and Anti-XPress

Expression

Mammalian

Mutation

Y271/273F

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-IP3KC

Plasmid

#32527

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

Insert

IP3KC (ITPKC Human)

Use

Tags

6xHIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-hB50

Plasmid

#18967

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican N-terminus (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Human brevican N-terminus aa 1-400

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Article

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 WT

Plasmid

#12181

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

V1467L

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mitoT

Plasmid

#174519

Purpose

Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

Depositor

Oleh Khalimonchuk

Article

Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

Insert

MitoT synthetic tether

Use

Tags

6xHis and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔPKA

Plasmid

#183452

Purpose

Expression of human AKAP79 lacking the PKA binding site (Δ391-400)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

aa391-400 deleted

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 KD

Plasmid

#12180

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Kinase dead: K1253M; V1467L

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3_2FLAG-AKAP79ΔCaM

Plasmid

#183453

Purpose

Expression of human AKAP79 lacking the calmodulin binding site (Δ79-86)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

aa79-86 deleted

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf6

Plasmid

#10834

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1

Plasmid

#10830

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 (ARF1 Bovine)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-ING4

Plasmid

#13289

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING4 (ING4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-CAML

Plasmid

#22284

Depositor

Erik Lillehoj

Article

Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

Insert

calcium-modulating ligand (CAMLG Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-ING5

Plasmid

#13290

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING5 (ING5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-hBnvy

Plasmid

#18969

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican uncleavable (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Mutation at the ADAMTS cleavage site ESE/SRG: S…

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 RL3Am

Plasmid

#109364

Purpose

Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_mScarlet-LAMP1

Plasmid

#185138

Purpose

Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

Depositor

Andrew York

Article

Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

Insert

Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

deleted amino acids 1-21 of LAMP1

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA-Sumo1 WT

Plasmid

#48966

Purpose

Expresses HA-Sumo1 WT in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA SF-HERC2 F2

Plasmid

#55785

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3_altPKA-Cα-mEGFP

Plasmid

#114132

Purpose

Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

18747 (Prkaca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-D2s-L-V1

Plasmid

#19967

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-N terminal Venus (DRD2 Aequorea Victoria, Human)

Use

Tags

Flag and N terminal Venus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-IP3KA

Plasmid

#32529

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KA (ITPKA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F1

Plasmid

#55784

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F3

Plasmid

#55786

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F6

Plasmid

#55789

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only