Addgene

We narrowed to 11,638 results for: pcdna

Showing: 2521 - 2550 of 11638 results

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pcDNA-Ikka-HA (K44M)

Plasmid

#23297

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

IKK alpha (Chuk Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

K44M

Promoter

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-WT-VLCAD-FLAG

Plasmid

#55823

Purpose

Expresses WT-VLCAD in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1_d77

Plasmid

#172588

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

deleted amino acids 1-76

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 wt

Plasmid

#27629

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 (Myc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp8

Plasmid

#157694

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 (-) hYAP 1-2delta

Plasmid

#124146

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsUNR_AA

Plasmid

#148239

Purpose

Mammalian Expression of HsUNR

Depositor

Elisa Izaurralde

Insert

HsUNR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) VSFP2.1

Plasmid

#16255

Depositor

Thomas Knopfel

Article

Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Insert

VSFP2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-Fc(IgA1)

Plasmid

#145154

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-C-terminal YPet

Plasmid

#128027

Purpose

Mammalian expression vector with C terminal YPet tag

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lunatic Fringe

Plasmid

#24962

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Lunatic Fringe (lfng Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Timothy Springer, Pontus Nordenfelt

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6-HA-AML1-ETO

Plasmid

#12508

Depositor

Dong-Er Zhang

Article

Peterson et al Blood. 2007 May 15;109(10):4392-8. Epub

Insert

HA-AML1-ETO (RUNX1T1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2v2.4-8his

Plasmid

#149664

Purpose

Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.4

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1

Plasmid

#32487

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1 (Gpd1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA6.MCV.cLT206.V5(CM2B4)

Plasmid

#28189

Depositor

Patrick Moore

Insert

MCPyV Large T antigen (206 wild-type strain)

Use

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR(T/A)

Plasmid

#118410

Purpose

Negative-control mutant for ExRai-CKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600uDelta13V

Plasmid

#18014

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CTLA4-mgSrtA

Plasmid

#125794

Purpose

for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CTLA4-mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB8

Plasmid

#19531

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

Plasmid

#169328

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2.4-sfGFP

Plasmid

#149666

Purpose

Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 1x (S1x)

Plasmid

#37003

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin (Syp Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

One copy of pHluorin inserted at site 184-185

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Use

Tags

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pY128 (pcDNA3.1-U6-huMb2Cpf1)

Plasmid

#92304

Purpose

Expression of humanized Mb2Cpf1 and crRNA

Depositor

Feng Zhang

Insert

huMb2Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/NS5-Mt-mut

Plasmid

#120830

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5 E218A "Mtase mut"

Use

Tags

V5

Expression

Mammalian

Mutation

E218A

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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