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Plasmid#60398PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C
Plasmid#60405PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeft2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpgk1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpxr1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsbtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Peno2-ACDH-Ttal1
Plasmid#127743PurposeYeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeno2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Pvma6-ACDH-Trps14a
Plasmid#127744PurposeYeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPvma6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Prpl28-ACDH-Tecm10
Plasmid#127745PurposeYeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrpl28Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Ptdh3-ACDH-Trpl3
Plasmid#127746PurposeYeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX-Slu7
Plasmid#111352Purposeprotein purificationDepositorInsertSLU7
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-Prp18
Plasmid#111354Purposeprotein purificationDepositorInsertPRP18
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mScarletI pDRF1-GW
Plasmid#168085PurposeExpresses Lifeact-mScarletI to visualize actin in yeastDepositorInsertLifeAct-mScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHZ1493
Plasmid#222102PurposeCloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mTq2 pDRF1-GW
Plasmid#168084PurposeExpresses Lifeact-mTq2 to visualize actin in yeastDepositorInsertLifeAct-mTq2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mScarlet pDRF1-GW
Plasmid#168086PurposeExpresses Lifeact-mScarlet to visualize actin in yeastDepositorInsertLifeAct-mScarlet
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
yomCherry pDRF1-GW
Plasmid#168087PurposeExpresses yomCherryDepositorInsertyomCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
AausFP1 pDRF1-GW
Plasmid#168088PurposeExpresses AausFP1DepositorInsertAausFP1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
yotagRFPT pDRF1-GW
Plasmid#168089PurposeExpresses yotagRFPTDepositorInsertyotagRFPT
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Prp2
Plasmid#111348Purposeprotein purificationDepositorInsertPRP2
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14b-Prp5
Plasmid#111347Purposeprotein purificationDepositorInsertPRP5
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
ymTq2-yeVenus pDRF1-GW
Plasmid#168076PurposeFRET fusion between ymTq2 and yeVenus for use in yeastDepositorInsertymTq2-yeVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarletI-KanMX6
Plasmid#136690PurposeTagging genes in Cterm with mScarletI in fission yeastDepositorInsertmScarletI
UseTagsmScarletIExpressionYeastMutationPromoterNoneAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yomTagBFP2-T2A-mCherry
Plasmid#205493PurposeExpression of yomTagBFP2-T2A-mCherry in yeastDepositorInsertyomTagBFP2-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
prs316 CUP-GFP
Plasmid#128052PurposeYeast expression vector for N terminal GFP fusionsDepositorTypeEmpty backboneUseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW Electra1-T2A-mCherry
Plasmid#205498PurposeExpression of Electra1-T2A-mCherry in yeastDepositorInsertElectra1-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only