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Showing: 4201 - 4250 of 18949 results

  2. NINJA TC

    Plasmid
    #159274
    Purpose
    Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).
    Depositor
    Nikhil Joshi
    Article
    Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    NINJA TC
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159274-plasmid-map-sequence-id-310699

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  6. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid
    #132389
    Purpose
    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132389-plasmid-map-sequence-id-270333

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  8. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

    Plasmid
    #84484
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    84484-plasmid-map-sequence-id-221389

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  14. pJW1586

    Plasmid
    #121057
    Purpose
    mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2-T^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized mKate2, and au…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121057-plasmid-map-sequence-id-235972

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  15. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

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  16. pAAV-syn-FLEX-axon-jYCaMP1s

    Plasmid
    #135421
    Purpose
    Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant
    Depositor
    Kaspar Podgorski
    Article
    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s
    Has Service
    AAV1
    Insert
    jYCaMP1s
    Use
    AAV
    Tags
    GAP43 axon targeting sequence
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135421-plasmid-map-sequence-id-268929

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  20. pJW1595

    Plasmid
    #121062
    Purpose
    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121062-plasmid-map-sequence-id-235983

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  22. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121059-plasmid-map-sequence-id-235964

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  25. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121061-plasmid-map-sequence-id-235980

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  33. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

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  34. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

    Plasmid
    #196895
    Purpose
    Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    2xHA-CAMSAP3 (Camsap3 Mouse)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196895-plasmid-map-sequence-id-392230

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  35. LeGO-iBSD-5LO-d4

    Plasmid
    #182743
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182743-plasmid-map-sequence-id-367810

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  36. LeGO-iBSD-5LO-p12

    Plasmid
    #182744
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform p12 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182744-plasmid-map-sequence-id-367805

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  37. LeGO-iBSD-5LO-E376Q

    Plasmid
    #182742
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase E376Q (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    enzymatically inactive 5-LO E376Q mutant, codon o…
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182742-plasmid-map-sequence-id-367803

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  38. LeGO-iBSD-5LO-d13

    Plasmid
    #182745
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182745-plasmid-map-sequence-id-367797

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  41. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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  42. pRS314-4M5.3

    Plasmid
    #45830
    Depositor
    Dane Wittrup
    Insert
    4M5.3
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    June 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    45830-plasmid-map-sequence-id-64346

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  43. pRS316-4M5.3

    Plasmid
    #45831
    Depositor
    Dane Wittrup
    Insert
    4M5.3
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    June 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    45831-plasmid-map-sequence-id-64348

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  45. pAG416GAL-EYFP

    Plasmid
    #201792
    Purpose
    Expresses EYFP from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    EYFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201792-plasmid-map-sequence-id-402623

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  47. pAG416GALL-ccdB-6stop

    Plasmid
    #203511
    Purpose
    Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203511-plasmid-map-sequence-id-402867

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  48. pAG416GAL10-ccdB-6stop

    Plasmid
    #203512
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203512-plasmid-map-sequence-id-402865

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  49. pAG416GALL-HIV-1_PR

    Plasmid
    #203493
    Purpose
    Expresses HIV-1 protease from a GALL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    HIV-1 protease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GALL
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203493-plasmid-map-sequence-id-402844

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  50. pCI-SMN2-skipping-GLuc

    Plasmid
    #218670
    Purpose
    Alternative splicing signaling: signal decrease with Ex7 inclusion increasing
    Depositor
    Jingxin Wang
    Insert
    SMN2 (SMN2 )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    218670-plasmid-map-sequence-id-450976

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Showing: 4201 - 4250 of 18949 results