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Tad-CBE(TCC1)

Plasmid

#235066

Purpose

Expresses Tad-CBE(TCC1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCC1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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SP_C2

Plasmid

#228158

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast (S. mikatae) MEL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q11129_MEL_SACMI:0-18

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

Plasmid

#235158

Purpose

Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/primary transcript with control siRNA

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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EW394 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8-FKBP (FLP-IN)

Plasmid

#236158

Purpose

Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a GS linker to FKBP1A, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF35(5-8)-ZNF250(1-4)-FKBP

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHBJT007

Plasmid

#225158

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-Y358A

Plasmid

#215890

Purpose

Expression of TRBP-Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-L326A

Plasmid

#215889

Purpose

Expression of TRBP-L326A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1397-DddtoxA-C

Plasmid

#191583

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1397-DddtoxA-C

Plasmid

#191584

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1397-DddtoxA-N

Plasmid

#191585

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1397-DddtoxA-N

Plasmid

#191586

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1333-DddtoxA-C

Plasmid

#191587

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NI_G1333-DddtoxA-C

Plasmid

#191588

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NN_G1333-DddtoxA-C

Plasmid

#191589

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1333-DddtoxA-C

Plasmid

#191580

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1333-DddtoxA-N

Plasmid

#191581

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NG_G1333-DddtoxA-N

Plasmid

#191582

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP703-P2A-GFP

Plasmid

#197158

Purpose

Expresses the protein of emiRFP703-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET29b-clbH-C-A2-PCP S1524A

Plasmid

#114158

Purpose

Expresses ClbH-C-A2-PCP S1524A

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

S-Tag, Thrombin site

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH with S…

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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