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Amph1-pmCherryN1

Plasmid

#27692

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Amphiphysin 1 (Amph Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Su9-EGFP

Plasmid

#23214

Depositor

David Chan

Publication

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Subunit 9 of the F0-ATPase

Tags

EGFP

Expression

Mammalian

Mutation

contains AA 1-69 of subunit 9

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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EGF-bio-His

Plasmid

#53340

Purpose

Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

EGF (EGF Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG vGlut1 mCherry

Plasmid

#105010

Purpose

Excitatory presynaptic marker

Depositor

Franck Polleux

Publication

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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GFP HDAC5 WT

Plasmid

#32211

Depositor

Reuben Shaw

Publication

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Publication

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p4xACO-Luc

Plasmid

#16533

Depositor

Bert Vogelstein

Publication

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

4xACO

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Str-Ii_SBP-mCherry-Golgin84

Plasmid

#65304

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmOrange

Plasmid

#37129

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

His

Expression

Bacterial

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

araB

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO human ULK1 shRNA 8

Plasmid

#27633

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

human ULK1 shRNA 8

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Publication

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Publication

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Publication

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDONR223-FLT4

Plasmid

#23923

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT4 (FLT4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Publication

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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LentiGuide Blast

Plasmid

#199622

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

Depositor

Danwei Huangfu

Publication

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSMP-Luc

Plasmid

#36394

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Firefly Luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
p(HA)HIF1alpha (P402A,P564A)

Plasmid

#52636

Purpose

mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

Depositor

Eric Huang

Publication

Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

Insert

HIF1alpha P402A, P564A (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

P402A, P564A

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PH-PLCD1(R40L)-GFP

Plasmid

#51408

Purpose

Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 40 mutated to leucine

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles