We narrowed to 5,818 results for: pCas

1. pCAGGS-CRY2-mCherry-MYPT169

   Plasmid

   #178526

   Purpose

   Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry-MYPT169
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-GBP1-10gly-Gal4DBD

   Plasmid

   #49438

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP1-Gal4 DNA binding domain fusion protein
   

   Expression

   Mammalian

   Mutation

   C98S (to increase protein solubility), Q3D

   Promoter

   CAG

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-mTagBFP2-T2A-iCre

   Plasmid

   #113837

   Purpose

   Monomeric TagBFP2 and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCre
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS D614 soluble Spike

   Plasmid

   #164652

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Tags

   HIS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-Cas13d-T2A-GFP(1-10)

   Plasmid

   #224566

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-GFP(1-10)
   

   Use

   CRISPR

   Tags

   T2A-split GFP(1-10)

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-Flag-hsDicer (D1709A)

   Plasmid

   #41588

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D1709A

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. PX429 SpCas9 N863A nickase

   Plasmid

   #68706

   Purpose

   SpCas9 N863A nickase

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   SpCas9 N863A nicking mutant
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-SpCas9_sgRNA-site1 (RTW443)

   Plasmid

   #160136

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-RFP-miR-Scrint

    Plasmid

    #19825

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    Scrambled miRNA
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-FLEX-rc[Chronos-GFP]

    Plasmid

    #59056

    Purpose

    Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-MCP-VPR-2AmScarlet

    Plasmid

    #121964

    Purpose

    Mammalian expression, Transcription regulation module

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Cas13d-T2A-Citrine

    Plasmid

    #224565

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-Citrine
    

    Use

    CRISPR

    Tags

    T2A-Citrine

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HIF1 alpha K532R

    Plasmid

    #21102

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Promoter

    CAG

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cmv d0 dPspCas13b-GGS-Sun

    Plasmid

    #119858

    Purpose

    Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-Sun
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-postASAP-p2a-ChrimsonR

    Plasmid

    #178794

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and ChrimsonR
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-GBP6-10gly-VPminx4

    Plasmid

    #50796

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP6-VPminx4 activation domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid

    #111508

    Purpose

    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    VPR-dSpCas9[StaPL(TI)]
    

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, and S122…

    Promoter

    PGK

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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