We narrowed to 5,653 results for: pCas

1. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-mTagBFP2-T2A-dre

   Plasmid

   #124832

   Purpose

   Monomeric TagBFP2 and dre recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-dre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAX APPs-751-alpha

   Plasmid

   #30149

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein - soluble (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   splice variant 751; amino acids 1–612

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-loxpSTOPloxp-lyn-NeonGreen

   Plasmid

   #175257

   Purpose

   membrane target protein labelling

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   Lyn membrane targeting motif
   

   Use

   Cre/Lox

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pCAG

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-nDsRedFL-miR9/9*/124

   Plasmid

   #177807

   Purpose

   The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-9/9*/124
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX551-miniCMV-SpCas9D10A nickase

   Plasmid

   #216735

   Purpose

   Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   T7 and mini-CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-pac-T2A-dre

   Plasmid

   #124833

   Purpose

   Puromycin resistance gene and dre recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-dre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS/XBB.1.5-RBD

    Plasmid

    #213038

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/XBB.1.5-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Bmpr2ΔKinase gRNA resistant

    Plasmid

    #163411

    Purpose

    Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted. gRNA-t…

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-CIBN-EGFP-KRasCT

    Plasmid

    #178527

    Purpose

    Expresses CIBN-EGFP-KRasCT in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CIBN-EGFP-KRasCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Gag-FKBP-Myc

    Plasmid

    #177909

    Purpose

    HIV Gag-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    HIV Gag (gag )
    

    Use

    Tags

    FKBP12 and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-Flag-hsDicer (K70A)

    Plasmid

    #41589

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K70A

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASP-SptP120-K55-HilA

    Plasmid

    #153329

    Purpose

    Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K55 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-PB1-AG(Y63L)-LOV2

    Plasmid

    #178876

    Purpose

    Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen(Y63L)-LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AzamiGreen: Y63L

    Promoter

    CAG

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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