We narrowed to 8,554 results for: sgRNA

1. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

   Plasmid

   #148859

   Purpose

   Mammalian Expression of HsXRN1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsXRN1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

   Plasmid

   #148857

   Purpose

   Mammalian Expression of HsPatL1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsPatL1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

   Plasmid

   #148858

   Purpose

   Mammalian Expression of HsTNRc6C-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRc6C-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBHM2646

   Plasmid

   #229530

   Purpose

   Carries mAID_opt tag, empty sgRNA, and NATR marker

   Depositor

   Hiten Madhani

   Insert

   mAID_opt - empty sgRNA - NATR
   

   Use

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPRv2_ZsG gRNA_4

   Plasmid

   #192688

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPRv2_ZsG gRNA_1

   Plasmid

   #192685

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiCRISPRv2_ZsG gRNA_2

   Plasmid

   #192686

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPRv2_ZsG gRNA_3

   Plasmid

   #192687

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJR98

    Plasmid

    #187239

    Purpose

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX458 eSp(1.1) V3

    Plasmid

    #226963

    Purpose

    CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Carol Gross, Stanley Qi, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP2

    Plasmid

    #79874

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into amyE

    Depositor

    Carol Gross, Stanley Qi, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWS082

    Plasmid

    #90516

    Purpose

    sgRNA entry vector

    Depositor

    Tom Ellis

    Insert

    Yeast sgRNA expression vector
    

    Use

    CRISPR; Yeast gap repair

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBHM2644

    Plasmid

    #229528

    Purpose

    Carries AID* tag, empty sgRNA, and NATR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - NATR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBHM2645

    Plasmid

    #229529

    Purpose

    Carries mIAA7_opt tag, empty sgRNA, and NATR marker

    Depositor

    Hiten Madhani

    Insert

    mIAA7_opt - empty sgRNA - NATR
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge