166,289 results

1. Epac-S-H326

   Plasmid

   #228242

   Purpose

   mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Epac-S-H327

   Plasmid

   #228243

   Purpose

   mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Epac-S-H328

   Plasmid

   #228244

   Purpose

   mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Epac-S-H329

   Plasmid

   #228245

   Purpose

   mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Epac-S-H332

   Plasmid

   #228250

   Purpose

   mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

   Insert

   mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUASTattB-degradALFA

   Plasmid

   #219663

   Purpose

   Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   degradALFA
   

   Expression

   Insect

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWN172

   Plasmid

   #215291

   Purpose

   Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   J23113

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC70189

   Plasmid

   #209450

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Promoter

   HvU3

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-GRAB_g5-HT3.0mut

   Plasmid

   #208712

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-MAST3_IDG-K

    Plasmid

    #198007

    Purpose

    Gateway destination clone of MAST3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAST3 (MAST3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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