We narrowed to 23,540 results for: promoter

1. pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE

   Plasmid

   #233047

   Purpose

   To express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' direction

   Depositor

   Jonathan Ploski

   Insert

   GFP and GluN2B
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

   Plasmid

   #219699

   Purpose

   Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag

   Depositor

   Jennifer Nemhauser

   Insert

   pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
   

   Expression

   Plant

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ1-g1g2-K2

   Plasmid

   #218217

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK7-g7g8-K8

   Plasmid

   #218220

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK3-g3g4-K4

   Plasmid

   #218218

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK5-g5g6-K6

   Plasmid

   #218219

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNSU299_RRT8

   Plasmid

   #166080

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of RRT8 (RRT8 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNSU299_RPL22A

   Plasmid

   #166079

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of RPL22A (RPL22A Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNSU299_YNR071C

   Plasmid

   #166081

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of YNR071C (YNR071C Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRP-iSL(MCS1+2)

    Plasmid

    #69245

    Purpose

    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGfa2(ABDx3)-nLac

    Plasmid

    #53133

    Purpose

    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells

    Depositor

    Michael Brenner

    Article

    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    -2163 to +47, with 3 copies of 3 copies of the gf…

    Promoter

    human Gfa2

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGfa(B3)-nLac

    Plasmid

    #53132

    Purpose

    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells

    Depositor

    Michael Brenner

    Article

    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    -2163 to +47, with 3 copies of the gfa2 B segment…

    Promoter

    human Gfa2

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti.pFOXA2.5'.GFP

    Plasmid

    #59407

    Purpose

    expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    FOXA2

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGV13 (CK2alpha K68M, CK2beta)

    Plasmid

    #27094

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha K68M (CSNK2A1 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA on CK2alpha and Myc on CK2beta

    Expression

    Mammalian

    Mutation

    K68M on CK2alpha

    Promoter

    bidirectional tet-responsive promoter and bidirec…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid

    #183080

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -124C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid

    #183081

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -146C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid

    #183079

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    Wildtype promoter, Exon 1 C7C (C21>T21) silent…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-ADP-MCS-FLAG

    Plasmid

    #192360

    Purpose

    Adipocyte-specific overexpression of genes

    Depositor

    Yifu Qiu

    Article

    Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

    Type

    Empty backbone

    Use

    AAV

    Tags

    3xflag

    Expression

    Mammalian

    Promoter

    adiponectin promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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