We narrowed to 13,228 results for: sequence

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EF1a_SPIB_P2A_Hygro_Barcode

Plasmid

#120483

Purpose

Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIB (SPIB Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-iRFP

Plasmid

#175842

Purpose

Express iRFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_PAX7_P2A_Hygro_Barcode

Plasmid

#120472

Purpose

Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

PAX7 (PAX7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-lck-smV5

Plasmid

#196423

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox

Tags

Lck

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Available Since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pHC01

Plasmid

#106900

Purpose

Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

Depositor

Jonathan Silberg

Article

Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

Inserts

LasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein

Expression

Bacterial

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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PM2-FRB-CFP

Plasmid

#67518

Purpose

FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence from human Lyn…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pFX-GalT-EGFP

Plasmid

#174471

Purpose

Express EGFP with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-mCherry

Plasmid

#159742

Purpose

Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_MYOG_P2A_Hygro_Barcode

Plasmid

#120465

Purpose

Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYOG (MYOG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b- mKate2-KDEL-pA

Plasmid

#109494

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b- mKate2-KDEL-pA

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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WNK2 K207A

Plasmid

#24570

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2` (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Mutation

K207A

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pFX-GalT-mCherry

Plasmid

#174473

Purpose

Express mCherry with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJC30X-BS(X)

Plasmid

#58958

Purpose

Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

Depositor

Thomas Fox

Article

Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.

Insert

Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code

Tags

First 8 codons of COX3

Mutation

GFP modified as described: CORMACK, B. P., R. H. …

Promoter

n/a

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pFX-H2B-mCherry

Plasmid

#175841

Purpose

Express mCherry with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Mutation

ARS sequence mutated to remove sapI site for dome…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pdUA-lacIN

Plasmid

#141079

Purpose

Plasmid vector for testing DNA binding proteins by the I-Block assay.

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Type

Empty backbone

Use

Vector for the i-block assay designed to study dn…

Expression

Bacterial

Promoter

Escherichia coli lacIN promoter

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_SIX1_P2A_Hygro_Barcode

Plasmid

#120476

Purpose

Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SIX1 (SIX1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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