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KG#65

Plasmid

#110933

Purpose

Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

unc-17 beta promoter
unc-54 3' control region

Expression

Bacterial

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 SP2

Plasmid

#67109

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SP2 (SP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pPromoterless-P2A

Plasmid

#165790

Purpose

Viral P2A part in promoter grammar, for building promoterless control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless-2A

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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plko-puromycin-shTRX1-259

Plasmid

#110921

Purpose

To knockdown Thioredoxin-1

Depositor

Priyamvada Rai

Article

Young et al Biochem Biophys Res Commun. 2010 Feb 12;

Insert

TRX1 (TXN Human)

Use

Lentiviral

Promoter

unknown

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6_Pfrm_BsMdh_BmHps_BmPhi

Plasmid

#129109

Purpose

Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

Depositor

Eleftherios Terry Papoutsakis

Article

Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

Insert

BsMDH BmHPS BmPHI

Expression

Bacterial

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGL2_177

Plasmid

#199109

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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Elk1 pShuttle

Plasmid

#121097

Purpose

Expression of full length ELK1 in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV0-OCT-HA-eGFP

Plasmid

#67479

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pHR Src deltaSH2/3-TagBFP

Plasmid

#171033

Purpose

TagBFP-tagged Src variant lacking SH2 and SH3 domains.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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KG#707

Plasmid

#110911

Purpose

Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

Insert

sup-1 minigene

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-HaAEP1

Plasmid

#159530

Purpose

Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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GPKAnls

Plasmid

#61094

Purpose

GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal

Depositor

Steven Green

Article

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p5xUAS

Plasmid

#165891

Purpose

Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xUAS

Expression

Insect

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p5xQUAS

Plasmid

#165818

Purpose

Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xQUAS

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F3 LUC

Plasmid

#22429

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F3 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Available Since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pVP16 AD

Plasmid

#165822

Purpose

VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

VP16 AD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GPKAnes

Plasmid

#61092

Purpose

GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal

Depositor

Steven Green

Article

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only