4,174 results

1. mCherry-Rab5CA(Q79L)

   Plasmid

   #35138

   Depositor

   Sergio Grinstein

   Article

   Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

   Insert

   Rab5a (RAB5A Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Constitutively Active- Q79L

   Promoter

   CMV

   Available Since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXAT2

   Plasmid

   #80494

   Purpose

   AAVS1 sgRNA expression vector

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 sgRNA-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-CYLD-WT

   Plasmid

   #15506

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   CYLD (CYLD Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVHA EED wt

   Plasmid

   #24231

   Purpose

   Mammalian expression of human EED with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EED (EED Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP GR

   Plasmid

   #47504

   Purpose

   Expresses glucocorticoid receptor in mammalian cells

   Depositor

   Alice Wong

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINE GFP nanobody

    Plasmid

    #49172

    Purpose

    Bacterial expression of GFP nano body

    Depositor

    Brett Collins

    Article

    Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

    Insert

    GFP nanobody
    

    Tags

    HHHHHH

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-TLR4-YFP

    Plasmid

    #13018

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 4 (TLR4 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. psfaRb-Tn5

    Plasmid

    #183638

    Purpose

    Expresses anti-rabbit nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti rabbit secondary nanobody fused to Tn5
    

    Expression

    Bacterial

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-EGFP-C4-Nrf2

    Plasmid

    #21549

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 IKKe flag

    Plasmid

    #26201

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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