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We narrowed to 18,450 results for: gateway

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Showing: 3441 - 3480 of 18450 results

  1. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid
    #121722
    Purpose
    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    osTir1-P2A-AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    9x myc (C-term on osTIR1)
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121722-plasmid-map-sequence-id-237078

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  2. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

    Plasmid
    #121831
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/LSD1 (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121831-plasmid-map-sequence-id-237385

    Enlarge

  5. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

    Enlarge

  7. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

    Enlarge

  8. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid
    #121833
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/Dnmt3a-3L (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121833-plasmid-map-sequence-id-237387

    Enlarge

  13. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62133-plasmid-map-sequence-id-101417

    Enlarge

  14. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121816
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121816-plasmid-map-sequence-id-237364

    Enlarge

  20. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121799
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121799-plasmid-map-sequence-id-237303

    Enlarge

  22. pMuLE ENTR U6-miR-30 R4-R3

    Plasmid
    #62117
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Expression
    Mammalian
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

    Enlarge

  24. KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR

    Plasmid
    #121829
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/VPR (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121829-plasmid-map-sequence-id-237383

    Enlarge

  25. KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core

    Plasmid
    #121832
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/p300core (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121832-plasmid-map-sequence-id-237386

    Enlarge

  26. pMuLE ENTR 7SK-miR-30 L5-L4

    Plasmid
    #62119
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Expression
    Mammalian
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

    Enlarge

  29. pMuLE ENTR U6 n.s shRNA L5-L4

    Plasmid
    #62138
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62138-plasmid-map-sequence-id-347565

    Enlarge

  32. pMuLE ENTR U6 n.s shRNA L1-R5

    Plasmid
    #62135
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62135-plasmid-map-sequence-id-352805

    Enlarge

  33. pMuLE ENTR SV40-hCas9 L5-L2

    Plasmid
    #62134
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62134-plasmid-map-sequence-id-101418

    Enlarge

  34. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid
    #121806
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121806-plasmid-map-sequence-id-237315

    Enlarge

  36. pMuLE ENTR CMV-hCas9 R4-R3

    Plasmid
    #62132
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62132-plasmid-map-sequence-id-101416

    Enlarge

  38. pMuLE ENTR 7SK-miR-30 L5-L2

    Plasmid
    #62120
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Expression
    Mammalian
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3441 - 3480 of 18450 results