We narrowed to 4,345 results for: Ado;

1. pEGFP Kind2 S181E

   Plasmid

   #105312

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181E

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP Kind2 S181A

   Plasmid

   #105307

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181A

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF1-MCS2-EF1-Puro-RGS7

   Plasmid

   #113728

   Purpose

   Express RGS7 in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-BE3-P2A-GFP-PGK-Puro

   Plasmid

   #110867

   Purpose

   Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-VQRRA-P2A-Puro

   Plasmid

   #110851

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

   Plasmid

   #184406

   Purpose

   yeast surface display of the SARS-CoV-2 Beta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   K417N+E484K+N501Y

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti PA-mCit-NL

   Plasmid

   #113452

   Purpose

   Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine-NanoLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBbC

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-HF1-PGK-Puro

   Plasmid

   #110857

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_HOXA1_P2A_Hygro_Barcode

   Plasmid

   #120451

   Purpose

   Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HOXA1 (HOXA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-VQRRA-PGK-Puro

    Plasmid

    #110858

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-Basic-Fascin-Promoter (-402-0)

    Plasmid

    #89823

    Purpose

    Promoter luciferase assay

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Fascin1 (FSCN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRT2-H2B-EGFP-V5-2A (IG157)

    Plasmid

    #99623

    Purpose

    To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    H2B-EGFP-V5
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSP1-mp53AS-6xMUT

    Plasmid

    #20904

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

    Insert

    p53AS (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    point mutation (Ser/Thr-Ala) of the 6 N-terminal …

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

15. EF1a_KLF12_P2A_Hygro_Barcode

    Plasmid

    #120498

    Purpose

    Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF12 (KLF12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-VRERRA-PGK-Puro

    Plasmid

    #110859

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SLC30A10-delta105-107

    Plasmid

    #65205

    Purpose

    Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta 105-107 amino acids

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

18. p-OiRS3GG

    Plasmid

    #98589

    Purpose

    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

    Depositor

    Lydia Contreras

    Article

    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

    Inserts

    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD and pLtetO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-VQR-P2A-Puro

    Plasmid

    #110848

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FRT3-Mb2-HA-mTfp1-2A (IG155)

    Plasmid

    #99621

    Purpose

    To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2-HA-mTfp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge