We narrowed to 7,572 results for: Lif

1. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

   Plasmid

   #202571

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-CALPAIN-7-mCherry

   Plasmid

   #180652

   Purpose

   Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-FLAG-APEX2-OPTN

   Plasmid

   #175760

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CVS-N2c-nl.EGFP-SypGFP

   Plasmid

   #172380

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + SypEGFP
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTY07

   Plasmid

   #83285

   Purpose

   human CENP-A N-terminally labeled with LSSmOrange

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   centromere protein A (CENPA Human)
   

   Use

   Retroviral

   Tags

   LSSmOrange

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PM-CaNAR2

   Plasmid

   #64730

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   Lyn-CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal targeting sequence from Lyn…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF(UG) hSyn SYT7alpha-HaloTag

   Plasmid

   #179715

   Purpose

   Lentiviral expression of mouse Syt7 with C terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-u(M)Cas9

   Plasmid

   #127384

   Purpose

   Expresses Cas9 at medium level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(M)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS-HsProm1s1-StayGold

   Plasmid

   #211333

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Kindlin

    Plasmid

    #186135

    Purpose

    For expression of recombinant kindlin in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    FERMT2 (FERMT2 Human)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1a-HygroR-T2A-TetOn3G

    Plasmid

    #179887

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    Hygromycin resistance

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Flp-DOG-NW

    Plasmid

    #75469

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Flp-DOG
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRXIP-GFP-FAPP(PHD)

    Plasmid

    #221748

    Purpose

    Stable expression of GFP-FAPP(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    FAPP (PLEKHA3 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–101

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. NK73

    Plasmid

    #176609

    Purpose

    To test bead engulfment of macrophages.

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    SNAP-CD86-FCERG (CD86 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDAS12342_PEAR-GFP_2in1

    Plasmid

    #177184

    Purpose

    PEAR-GFP plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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