We narrowed to 2,598 results for: pCas

Showing: 1721 - 1740 of 2598 results

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hAID-BE3 (pRZ352)

Plasmid

#131316

Purpose

CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Publication

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL680 CHYRON3 integration construct

Plasmid

#126448

Purpose

To integrate the CHYRON3 locus at AAVS1 in human cells.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON3 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA1

Plasmid

#89719

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Publication

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA1 (IRF8 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAK004

Plasmid

#233641

Purpose

For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.

Depositor

Zak Swartz

Publication

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

eSpCas9(1.1) with c-terminal VitelloTagA

Tags

6-His

Expression

Bacterial

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBRC1

Plasmid

#183064

Purpose

Base editing plasmid for Pseudomonas chlororaphis

Depositor

Xuehong Zhang

Publication

Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

Insert

eSpCas9ppD10A

Use

CRISPR and Synthetic Biology

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_449

Plasmid

#179093

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Publication

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpG

Use

CRISPR and Lentiviral

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_miniABEmaxNG(GG)

Plasmid

#187062

Purpose

miniABEmax(GG)-SpCas9-NG

Depositor

Renzhi Han

Publication

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax(GG)-SpCas9-NG

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFH55

Plasmid

#125814

Purpose

Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-PmCDA, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

Plasmid

#135013

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide

Depositor

Yosef Shaul

Publication

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3x Flag, 3xFLAG (N terminal on insert), NLS, and …

Expression

Mammalian

Mutation

126aa domain from HSV-1 UL12 fused to the N-termi…

Promoter

CBh

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFH52

Plasmid

#128181

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH66

Plasmid

#131765

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAGT9569

Plasmid

#219431

Purpose

Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

Depositor

Tom Schreiber

Publication

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

PapE-4LF2

Expression

Plant

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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hs-lambda btl

Plasmid

#17581

Depositor

Denise Montell

Publication

Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

Insert

lambda-btl (btl Fly)

Expression

Insect

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

D10A mutation; NLS fusion

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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GluA3 (px330:2-guideCas9)

Plasmid

#169424

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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CP1300-ABEmax

Plasmid

#119810

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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CP1249-CBEmax

Plasmid

#119804

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1249) -UGI-UGI

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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PDG458 eSp(1.1) V3

Plasmid

#226964

Purpose

CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAGT9054

Plasmid

#219430

Purpose

Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

Depositor

Tom Schreiber

Publication

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)

Use

CRISPR; Moclo compatible level 1 module (position…

Tags

PapE-4LF2

Expression

Plant

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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