We narrowed to 3,243 results for: phage

1. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

   Plasmid

   #160582

   Purpose

   35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   attR:P35S:TMtb:attL
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT175_GalL_A3A(Y130F)Δ186-BE3

   Plasmid

   #145124

   Purpose

   Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)Δ186-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(Y130F; 1-186AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

   Plasmid

   #133797

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1(T16A)
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   T16A

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

   Plasmid

   #133799

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized Leu-AcrIIA1
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

   Plasmid

   #133800

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized Eriv-AcrIIA1
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTSlb-R756S

   Plasmid

   #60730

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSara-R756S

   Plasmid

   #60725

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSara-Q758C

   Plasmid

   #60724

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. C86m

   Plasmid

   #120997

   Purpose

   MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   X174E
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. C115m

    Plasmid

    #121014

    Purpose

    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    TP901 integrase
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE6e

    Plasmid

    #207855

    Purpose

    Mammalian expression of PE6e prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6e
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, K918A, K775R)

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-T7-6h-GST-α-Synuclein

    Plasmid

    #225224

    Purpose

    Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    GST
    α-Synuclein
    

    Tags

    6xHis Tag and TEV Cleavage Site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET42b-BE3

    Plasmid

    #87437

    Purpose

    Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiCRISPRv2Cre

    Plasmid

    #82415

    Purpose

    Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    Cre Recombinase
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Mutation

    Mutated BsmBI cut site

    Promoter

    EFS (P2A)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET42b-HF-BE3

    Plasmid

    #87438

    Purpose

    Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC059-HisMS2_PLP_NucPhos_pac

    Plasmid

    #155039

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Nucleocapsid Phosphoprotein
    

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available Since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCMV-HF-BE3

    Plasmid

    #87439

    Purpose

    Expresses HF-BE3 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
    

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available Since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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