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mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-iATPSnFR1.0

Plasmid

#102556

Purpose

expresses cytoplasmic ATP sensor in neurons

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 eta

Plasmid

#116887

Purpose

Expresses HA-tagged human 14-3-3 eta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 eta (YWHAH Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-LC3-YFP

Plasmid

#99571

Purpose

Lentiviral expression of YFP-tagged LC3

Depositor

Edward Campbell

Article

Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

Insert

LC3 (MAP1LC3B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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