We narrowed to 3,182 results for: actin

1. pCA-G-intron(Neo)-T(LNL)

   Plasmid

   #36907

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   Neo
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdEos-VASP-5

   Plasmid

   #57685

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   VASP
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV GFP

   Plasmid

   #213685

   Purpose

   Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

   Depositor

   Thomas Kukar

   Article

   Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

   Insert

   Twin-Strep GFP
   

   Use

   AAV

   Tags

   Twin-Strep

   Expression

   Mutation

   Promoter

   cytomegalovirus enhancer/chicken β-actin promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1-lifeact-oROS-HT

   Plasmid

   #216420

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   lifeact-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Combined γB2FL-FRET probe

   Plasmid

   #209094

   Purpose

   mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

   Depositor

   Takeharu Nagai

   Article

   Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Combined γB2ΔICD-FRET probe

   Plasmid

   #209093

   Purpose

   mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav2.2 pCAGGS

   Plasmid

   #206101

   Purpose

   expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cav2.2 HA pCAGGS

   Plasmid

   #206052

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

   Plasmid

   #168254

   Purpose

   "label stable F-actin under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

    Plasmid

    #136912

    Purpose

    DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    pCAG-DmrC-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid

    #182680

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pExpreS2-1-CR-PA

    Plasmid

    #175445

    Purpose

    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    BiP Secretion Peptide and Thrombin-Protein A

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

    Plasmid

    #168253

    Purpose

    "label stable F-actin; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Utrophin-GFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTone-gata2aECE-EGFP

    Plasmid

    #132974

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Inserts

    eGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    

    Use

    Unspecified; Transposon-mediated

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

    Plasmid

    #89060

    Purpose

    AAV-gRNA cloning vector with GFP reporter

    Depositor

    William Lagor

    Article

    Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

    Insert

    EmGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin with partial CMV enhancer

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Use

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TK-GFP-Nluc-P2A-neo-TK

    Plasmid

    #134896

    Purpose

    Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Insert

    TK-GFP-Nluc-P2A-Neo-TK
    

    Use

    Cryptosporidium expression

    Tags

    Expression

    Mutation

    Promoter

    Cryptosporidium actin and enolase

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHLmMBP-3

    Plasmid

    #72345

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-N-Blast-mNeon-ACTB

    Plasmid

    #207751

    Purpose

    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExpreS2-1-C3-10H-SIII

    Plasmid

    #175444

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-10His-TwinStrep and BiP Secretion Peptide

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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