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Addgene
Showing: 1741 - 1760 of 2899 results
  1. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #219653
    Purpose
    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus
    Depositor
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)
    Expression
    Mammalian
    Mutation
    Promoter
    nEf Addgene # 149296
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

    Plasmid
    #202717
    Purpose
    Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging
    Depositor
    Inserts
    Myr-Pink Flamindo
    EGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pJL30

    Plasmid
    #174064
    Purpose
    Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
    Depositor
    Insert
    pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1741 - 1760 of 2899 results