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Plasmid#221776PurposeExpresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cellsDepositorInsertpCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Plasmid#219653PurposeGeneral mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark VenusDepositorInsert2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
UseAAVTags2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)ExpressionMammalianMutationPromoternEf Addgene # 149296Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB
Plasmid#219557PurposePM-targeted iLID-LOVPLDDepositorInsertlyn10-iLID-LOVPLD
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C+F-ArchT-GFP
Plasmid#209700PurposeExpresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinasesDepositorInsertArchT-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ca-LiPPI-CLAPon1.0 (TEVp)
Plasmid#213409PurposeLight-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)DepositorInsertCaM-V5-TEVp
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh-SOS
Plasmid#208278PurposeExpresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh-EGFR
Plasmid#208281PurposeExpresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-EGFR
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-shNC
Plasmid#206356PurposeAAV plasmid expressing non-targeting control shRNA in photoreceptorsDepositorInsertNon-targeting control shRNA
UseAAVTagsExpressionMutationPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC
Plasmid#202717PurposeMammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imagingDepositorArticleInsertsMyr-Pink Flamindo
EGFP-bPAC
UseLentiviralTags10xHis and mycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-eLOV-TEVcs-Gal4
Plasmid#173119PurposeExpresses CCR6 eLOV receiver construct in DIPGDepositorInsertCCR6-eLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV CCR6-eLOV-TEVcs-Gal4
Plasmid#173113PurposeExpresses CCR6 receiver construct in mammalian cellsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS
Plasmid#192607PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuSDepositorInsertLexA-CIB1-biLINuS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-eGFP-P2A-CRY2-VPR
Plasmid#192610PurposeLentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.DepositorInserteGFP-P2A-CRY2PHR-VPR
UseLentiviral and Synthetic BiologyTagseGFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP01
Plasmid#186260PurposesfGFP under light light responsive PEL222 promoterDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBAR-Cry2PHR-WH2
Plasmid#176605PurposeCry2PHR with N-terminal IBAR domain and C-terminal WH2 domainDepositorInsertIBAR-Cry2PHR-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cry2PHR-IBAR-WH2
Plasmid#176603PurposeCry2PHR with C-terminal IBAR and WH2 domainsDepositorInsertCry2PHR-mCherry-IBAR-WH2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL30
Plasmid#174064PurposeEncodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.DepositorInsertpGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (L)
Plasmid#170624PurposeFor the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539EDepositorInsertLyn11-myc-LOV2GIVe (L)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only